摘要
目的:观察琼脂培养MTT法测定卵巢癌对顺氯氨铂(DDP)体外敏感性与临床疗效的关系。方法:用琼脂培养MTT法对26例卵巢癌患者的癌组织标本行药敏测定,同时观察DDP对卵巢癌治疗的临床疗效。结果:卵巢癌接种活细胞数与光密度(OD值)在一定范围内呈线性相关(r=0.9728,P<0.01)。临床缓解组DDP对卵巢癌细胞的相对抑制率(73±5)明显较临床无效组(36±9)高(P<0.01)。卵巢癌对顺铂体外敏感性与临床疗效相关,体内外阳性符合率是88%(16/18),阴性符合率为100%(8/8),总符合率是92%(2/26)。
To observe the correlation between drug sensitivity in vitro and clinical response of patients with ovarian cancer treated with cisplatin. Methods: The MTT colorimetric assay of agar culture was used to test the chemosensitivity of 26 fresh ovarian cancer samples. Therapeutic effect of cisplatin on the 26 patients with ovarian cancer was also abserved. Results: Linear regression analysis comparing the results of OD versus viable cells of ovarian cancer plated revealed excellent correlations (r=0.9728, P<0.001)。 The relative inhibitory rate of cisplatin of 16 clinical remission by the method (73±5) comparing with that of 10 no clinical response (36±9) is different significantly (P<0.01). Of the 26 specimens, 18 were sensitive to cisplatin in vitro had 16 clinical remission, and 8 cases resisitant to cisplatin showed no clinical response. The positive coincidence rate is 88 percent (16/18). The negative accordance rate is 100 percent (8/8). The total coincidence rate is 92 percent (2/26). Conclusion: The MTT colorimetric assay of agar culture can possibly predict drug sensitivity of human tumor clinically.
关键词
琼脂培养MTT法
顺氯氨铂
卵巢癌
治疗
the MTT assay of agar culture
ovarian cancer
cisplatin
drug therapy.