摘要
目的检测不同化疗敏感程度的卵巢上皮癌组织中P53基因的表达,分析其与化疗耐药的关系。方法 80例新鲜卵巢上皮癌组织均由手术中取得,采用ATP-TCA技术检测卵巢癌组织对八种化疗药物的体外敏感性。采用免疫组化SP法检测80例组织中P53基因的表达情况。结果对多西他赛化疗耐药的卵巢癌组织中,P53基因表达阳性率显著高于化疗敏感的组织(P=0.003),在对紫杉醇、卡铂及依托泊苷耐药的组织中其表达阳性率也显著升高(P<0.05)。结论研究结果提示:P53基因的高表达与卵巢癌化疗耐药相关,P53基因可能作为卵巢上皮癌化疗耐药性的预测指标。
Objective：The expression of P53 in ovarian epithelial cancer tissues with different chemosensitivity was examined to investigate the association between its expression and chemoresistance. Methods：Tissues were collected from 80 patients who underwent cytoreductive surgery. Viable ovarian cancer cells obtained from malignant tissues were tested for chemosensitivity using ATP-TCA. Immunohistochemical SP method was uesd to detect the expression of P53 in 80 ovarian epithelial cancer tissues. Results：The tissues resistant to docetaxel showed significantly higher P53 expression than sensitive one（P =0.003）. The same results were detected in paclitaxel,carboplatin and etoposide（P〈0.05）. Conclusion：The results indicated that the P53 overexpression is correlated to chemoresistance in ovarian carcinoma tissues. P53 protein expression may be used as a prediction of chemoresistance.
出处
《中国优生与遗传杂志》
2015年第12期18-19,31,共3页
Chinese Journal of Birth Health & Heredity
基金
首都卫生发展科研专项项目:"筛选卵巢癌化疗耐药标志物及其临床检测应用的研究"项目资助(首发2011-2008-05)
关键词
卵巢癌
P53
化疗耐药
Ovarian cancer
P53
Chemoresistance
