摘要
目的建立广州地区汉族人群人类血小板抗原(HPA)1—6、9及15系统和HLA-Ⅰ抗原基因分型库,探讨其基因多态性的分布,为有效避免血小板输注同种免疫的发生提供实验基础。方法随机抽取广州地区无血缘关系的健康机采血小板无偿献血者(均为汉族)的血样805份,采用PCR-SSP方法做HPA-1—6、9及15系统基因分型,利用Luminex-SSO方法做HLA-A、B、Cw抗原分型。结果HPA的基因频率分别为HPA-1a0.998、1b0.002,2a0.952、2b0.048,3a0.553、3b0.447,4a0.999、4b0.001,5a0.976、5b0.024,6a0.982、6b0.018,9a1.000、9b0.000,15a0.518、15b0.481;HPA-3、15a/b对偶抗原有aa、ab和bb 3种表型,HPA-1、2和4—6未发现bb表型,HPA-9未发现ab、bb表型;HPA-3a、3b,15a、15b的不配合率最高。经χ2检验,实验结果符合Hardy-Weinberg遗传定律。HLA-Ⅰ抗原基因频率较高的表达有A*02 0.286A*240.162A*11 0.323B*46 0.147B*75 0.100C*01 0.177C*03 0.289C*07 0.179。结论广州地区汉族HPA-1—6、9及15系统基因频率分布和HLA-Ⅰ类抗原分布存在明显的多态性,与其它资料相比显示出种族和地域性差异;建立HPA-1—6、9及15系统和HLA-Ⅰ抗原基因分型库对于临床输血有重要意义。
Objective To establish eight human platelet antigen systems and HLA-Ⅰ antigen donor bank, and to determine the gene frequencies of human platelet antigen （HPA） and HLA-Ⅰ in Guangzhou area. Methods A total of 805 blood samples from Chinese Han voluntary platelet donors were included in this study. PCR-SSP was used to detect single-nucleotide polymorphism in HPA systems. Luminex -SSO was used to detect the HLA-Ⅰ antigens. Results The distribution of HPA 1,2,3,4,5,6,9,15 was in Hardy-Weiberg equilibrium among study subjects. Allele frequencies of 0.998 and 0.002 were observed for HPA 1 a and 1 b, 0. 952 and 0. 048 for ttPA 2a and 2b, 0. 553 and 0. 447 for HPA 3a and 3b, 0. 999 and 0.001 for HPA 4a and 4b, 0. 976 and 0. 024 for HPA 5a and 5b, 0.982 and 0.018 for HPA 6a and 6b, 1 and 0 for HPA 9a and 9b, 0.518 and 0. 481 for HPA 15a and 15b. The high frequency HLA-I alleles were A^＊ 02, 0. 286 ; A^＊ 24, 0. 162;A^＊11,0.323; B^＊46,0. 147; B^＊ 75,0. 100;C^＊01,0. 177; C^＊ 03,0.289; C^＊ 07,0. 179. Conclusions This study confirmed the ethnic and territoria difference of HPA and HLA-Ⅰ. The establishment of HPA and HLA-Ⅰmatched plateletpheresis donor registry is helpful in the improvement in platelet transfusion.
出处
《中国输血杂志》
CAS
CSCD
北大核心
2009年第4期274-277,共4页
Chinese Journal of Blood Transfusion
基金
广州市医药卫生科技项目(编号:2008-YB-136)
广州市医药卫生科技重点项目资助课题(编号:2003-DZi-09)
