摘要
利用已构建的包含287个标记位点的遗传图谱的大白菜DH群体,采用多模型QTL作图的方法,通过苗期人工接种TuMV-C4株系对来自抗病亲本Y195-93和感病亲本Y177-12的DH群体的TuMV抗性进行QTL分析。共检测到3个QTLs,分别位于R03、R04和R06连锁群上,解释的表型变异在10.5%～21.9%之间,3个QTLs可解释40.0%的表型总变异。其中位于R04上命名为Tu-2的QTL解释的表型变异最高,为21.9%,其余两个位于R03和R06上的QTLs,分别解释10.5%和14.5%的表型变异。
One hundred and eighty-three doubled haploid （DH） lines derived from the cross of two Chinese cabbage [Brassica campestris L. ssp. pekinensis （ Lour. ） Olssom ] DH lines, Y177-12 （TuMV-susceptible） and Y195-93 （TuMV-resistant）, were used to map quantitative trait loci （QTL） related to TuMV resistance. TuMV resistance was evaluated for young seedlings by inoculating true leaves with TuMV-C4 strain. Based on the reported genetic map with 287 loci, 3 QTLs were detected for TuMV resistance, localizing on linkage groups R03, R04 and R06, respectively. The most significant of the identified QTL locates on R04, named Tu-2, explaining 21.9% of the phenotypic variance. The other two QTLs mapped on R03 and R06, explaining 10. 5% and 14.5% of the phenotypic variance, respectively. The three QTLs together explained 40. 0% of the TuMV resistance variance.
出处
《园艺学报》
CAS
CSCD
北大核心
2009年第5期731-736,共6页
Acta Horticulturae Sinica
基金
国家‘863’计划项目(2008AA102148)
‘十一五’国家科技支撑计划子课题项目(2006BAD01A7-1-06)
河南省重大科技攻关项目(072101110400)
关键词
大白菜
TUMV
QTL定位
DH群体
Chinese cabbage
Turnip mosaic virus （TuMV）
QTL mapping
DH population
