摘要
目的研究RNA干扰抑制结肠癌移植瘤模型VEGF-C表达和抗肿瘤淋巴管生成的有效方法。方法建立结肠癌裸鼠皮下移植瘤模型并随机分为3组(n=8):腺病毒(Ad5F35-VEGF-C-siRNA-EGFP)组、siRNA组和PBS组,对肿瘤进行原位注射,记录各组肿瘤体积变化,免疫组织化学法检测肿瘤内微血管密度(MVD)和淋巴管密度(LVD)。结果VEGF-C干扰能抑制肿瘤生长,且腺病毒组比siRNA组生长更慢。同时免疫组化显示各组MVD值的差异无统计学意义(P>0.05),siRNA组、腺病毒组和PBS组相比,LVD值的差异有统计学意义(P<0.05),LVD值发生不同程度地降低,且腺病毒组降低更显著(P<0.05)。结论病毒介导siRNA干扰VEGF-C的表达是抗肿瘤淋巴管生成的有效方法。
Objective To study the effective methods of VEGF-C blockade and antilymphangiogenie treatment in nude mice LOVO cells s.c. xenograft model by RNA interference. Methods The nude mice model was divided randomly into 3 groups （n=8）：the Ad5F35-VEGF-C-siRNA-EGFP group, siRNA group, PBS control group. After injection was administered intratumorally in each group the accumulation in tumor volume in each group was obertained. The status of angiogenesis and lymphangiogenesis were examined by immuno-histochemistry （IHC） staining with anti-CD31 monoclonal antibody and anti-LYVE-1 monoclonal antibody.The micro-vascular density （MVD） and lymphatic density（LVD） value was caculatedl Results VEGF-C blockade by RNAI inhibited the tumor growth, and the tumor growth in the AdSF35-VEGF-C-siRNA-EGFP group was slower than those in the siRNA group,there was no obviously variance in MVD value between the interference group and control group （P〉0.05）. There was varicance in LVD value between them （P〈0.05）, value in the AdSF35-VEGF-C-siRNA-EGFP group was the lowest. Conclusion The adenovirus-mediated VEGF-C-siRNA can significantly inhibit growth and lymphangiogenesis of human colorectal cancer in nude mice model.
出处
《苏州大学学报（医学版）》
CAS
北大核心
2009年第1期56-58,共3页
Suzhou University Journal of Medical Science
基金
苏州大学医学重点发展基金资助项目(2006-21-10)
