摘要
为研究小麦品种的抗寒机理,以东农冬麦1号为材料,根据连续三年的冬小麦低温驯化及越冬期的气温资料,当小麦长至6叶期时,设定不同温度处理,分析不同温度下植株地下茎处蛋白质的变化。结果表明,4个降温处理`中小麦植株地下茎处共有17个蛋白点发生显著变化。在这4次降温过程中重复出现的8蛋白点分别参与26 S蛋白水解途径、呼吸代谢、三羧酸循环、叶绿素合成,其中有一个位点是抗性蛋白。通过这8个蛋白点在不同温度下表达量的变化及蛋白点的功能对东农冬麦1号地下茎处抗寒性做了初步的推测。
The seedlings with 6 leaves of winter wheat cultivar ＂Dongnongdongmai 1＂ were treated in 5 combinations of temperature/ days based on temperature data for low temperature acclimation and over- winter period of winter wheat in 3 years, to reveal the change of proteins expressed in winter wheat cultivar ＂Dongnongdongmai 1＂ under different low temperature treatments. The changes of proteins in the seedling stems under soil surface were observed in different temperature treatments, and the results showed that there were significant changes in 17 protein spots by 4 low temperature treatments and 8 reappearing spots of those proteins were picked up for spectrum analysis. The 8 proteins were attributed to the processes of 26S protein dehydration, respiration, tricarhoxylic acid cycle, chlorophyll synthesis, glyoxylate cycle, respectively, and of which one spot was cold resistant protein. Some speculations on the frozen resistance of the cultivar was proposed preliminarily by the amount changes and functions of the 8 proteins under different low temperature degrees.
出处
《麦类作物学报》
CAS
CSCD
北大核心
2009年第3期484-490,共7页
Journal of Triticeae Crops
基金
东北农业大学创新团队项目(cxz003)
关键词
冬小麦
低温
地下茎
差异蛋白
Winter wheat
Low temperature
Stem under soil surface
Differentially expressed proteins
