摘要
目的研究塞克硝唑苯甲酸酯体外抗阴道毛滴虫的效果。方法以肝浸汤培养基培养阴道毛滴虫,将滴虫混悬液接种于96孔培养板中,分塞克硝唑苯甲酸酯组、塞克硝唑组、甲硝唑组、不加药物的对照组及空白组,通过四甲基偶氮唑盐微量酶反应比色法(MTT法)观察塞克硝唑苯甲酸酯的体外抗滴虫效果。另将滴虫混悬液接种于试管中,分塞克硝唑苯甲酸酯组、塞克硝唑组、甲硝唑组和不加药物的对照组,通过计数法观察塞克硝唑苯甲酸酯的体外抗滴虫效果。结果培养24h后,塞克硝唑苯甲酸酯浓度为0.15、0.31、0.63、1.25、2.50、5.00、10.0及20.0μg/ml时呈浓度依赖性地抑制滴虫增殖(t=9.02,P<0.01),MTT法相对抑制率分别为14.6%、28.7%、31.3%、60.4%、89.0%、89.2%、95.6%和100%;计数法相对抑制率分别为18.2%、31.1%、39.7%、68.8%、84.6%、90.1%、94.6%和100%。培养6～24h,呈时间依赖性地抑制滴虫增殖。体外抗阴道毛滴虫的最低杀灭浓度为20μg/ml,最低抑制浓度为0.15μg/ml。结论塞克硝唑苯甲酸酯具有较强的体外抗滴虫效果。
Objective To research the trichomonacidal effect of secnidazole benzoate in vitro. Methods Trichomonas vaginalis was cultured in liver extract medium in 96-well microplate. The culture suspension of Trichomonas vaginalis was divided into four groups： secnidazole benzoate, secnidazole, metronidazole and control, with medium as blank control. MTT colorimetric assay was applied to determine the inhibitory effect of secnidazole benzoate on the proliferation of Trichomonas vaginalis. The culture suspension was transfered into test tubes and divided into same groups to observe inhibitory effect by the classical microscopic counting method. Results After 24 h incubation, the proliferation of the parasites was concentration-dependent by secnidazole benzoate（t=9.02, P〈0.01）at the concentration ranges from 0.15 μg/ml to 20.0 μg/ml with a relative inhibition rate（%）of 14.6,28.7,31.3,60.4,89.0,89.2,95.6,and 100.0 for MTT colorimetric assay, and 18.2,31.1,39.7,68.8,84.6,90.1,94.6,and 100.0 for counting method,respectively. In the period of 6-24 h incubation, the inhibition was in a time-dependent manner. The minimum sterilizing concentration and the minimum inhibitory concentration were 20 μg/ml and 0.15 μg/ml respectively. Conclusion Secnidazole benzoate shows a similar trichomonacidal effect to metronidazole and secnidazole.
出处
《中国寄生虫学与寄生虫病杂志》
CAS
CSCD
北大核心
2009年第2期111-114,共4页
Chinese Journal of Parasitology and Parasitic Diseases
关键词
塞克硝唑苯甲酸酯
阴道毛滴虫
抗毛滴虫效果
MTT法
Secnidazole benzoate
Trichomonas vaginalis
Anti-trichomonas effect
MTT colorimetric assay
