摘要
目的探讨绿脓菌素（PCN）对人气道上皮细胞株（NCI-H292细胞）表达IL-8的诱导作用及通过蛋白激酶C（PKC）和核因子κB（NF-κB）的调控机制。方法采用ELISA法对PCN诱导NCI-H292细胞分泌IL_8进行分析，应用Western blot检测NF-κB的蛋白表达，观察PKC和NF-κB阻断剂对IL-8表达的影响。结果PCN可促进NCI-H292细胞IL-8的分泌，而且具有明显的量效关系。PKC阻断剂钙感光蛋白（Cal C）及NF-κB阻断剂二硫代氨基甲酸吡咯烷（PDTC）均能抑制IL-8的表达（P〈0．01）。PCN能直接诱导并迅速活化NF-κB，60～90min表达明显。结论PCN可能通过PKC信号通路促进NF-κB的活化，从而启动IL-8的高效表达。
Aim To study IL-8 expression of human airway epithelium-like NCI-H292 cells directly induced by Pyocyanin and its mechanism through protein kinase C （PKC） and nuclear factor-kappa B （NF-κB）. Methods ELISA methods were performed to test the expression of IL-8 in NCI-H292 cells infected by Pyocyanin. Western blot was employed to examine the expression of NF-κB protein. In addition, NCI-H292 cells were cultured together with PKC inhibitor, calphostin C or NF-κB inhibitor, pyrrolidine dithiocarbamate （PDTC）, respectively before Pyocyanin infection, then the expression of IL-8 and NF-κB was assayed using the above methods. Results Pyocyanin was able to induce IL-8 cells and had marked protein secretion in NCI-H292 concentration-dependent relations. Pyocyanin could also significantly promote the activation of NF-κB, which peaked at 60 ～ 90 min. PDTC and calphostin C could significantly decrease the activation of NF-κB and the expression of IL-8. Conclutions Pyocyanin can induce IL-8 production. The direct induction of Pyocyanin can promote the activation of NF-κB by PKC signal pathway, then cause the expression and secretion of IL-8.
出处
《中国药理学通报》
CAS
CSCD
北大核心
2009年第5期686-689,共4页
Chinese Pharmacological Bulletin
基金
辽宁省教育厅资助项目(No2008391)
