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电催化测定烟酰胺腺嘌呤二核苷酸——以聚亚甲基蓝-碳纳米管修饰电极作为工作电极 被引量:2

Electro-catalytic Determination of Nicotinamide Adenine Dinucleotide Using PMB-SWCNT′s/GCE as Working Electrode
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摘要 制备了聚亚甲蓝-碳纳米管修饰的玻碳电极(PMB-SWCNT’s/GCE),并研究了烟酰胺腺嘌呤二核苷酸(NADH)在此电极上的电化学行为。结果表明:此修饰电极对NADH表现出协同电催化作用和较好的响应性能。在pH7.0的磷酸盐缓冲溶液中可观察到NADH氧化峰电位的正移及其峰电流的增加,当NADH的浓度在5.0×10^-6~7.0×10^-4mol·L^-1范围内,与相应的氧化峰电流值之间存在线性关系。方法的检出限(3s/N)为1.4×10^-6mol·L^-1,所提出的方法应用于生物材料样品中NADH的直接测定,测得方法的平均回收率为99.5%。 Preparation of polymethylene blue, single-wall carbon nanotubes modified glassy carbon electrode (PMB-SWCNT' s/GCE) was described and the electrochemical behavior of nicotinamide adenime dinucleotide (NADH) at this modified electrode was studied. It was shown that the modified electrode behaved with synergisticcatalytic reaction and with good responese ability toward NADH. In an aqueous PBS of pH 7. 0, a positive shift of oxidation peak potential of NADH and an increase of peak current were observed, Linear relationship between values of the oxidation peak current and concentration of NADH was obtained in the range of 5. 0 × 10^-6 - 7. 0 × 10^-4mol · L^-1, Detection limit (3S/N) of the method obtained was 1.4 ×10 ^-6mol· L^-1 The proposed method was satisfactorily used to determine NADH in samples of biological materials, giving average recovery of 99.5%.
出处 《理化检验(化学分册)》 CAS CSCD 北大核心 2009年第5期533-536,共4页 Physical Testing and Chemical Analysis(Part B:Chemical Analysis)
基金 高等教育博士专项基金(SRFDP) 湖南省科技厅项目基金(2006FJK3182)资助
关键词 聚亚甲蓝-单壁碳纳米管 修饰玻碳电极 烟酰胺-腺嘌呤二核苷酸 协同电催化作用 Polymethylene Blue-single-wall carbon nanotubes Modified glassy carbon electrode Nicotinamide adenine dinucleotide Synergistic electro-catalysis
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