摘要
研究环芬化合物修饰电极的制备、电化学性质及对NADH的电催化作用。用光谱电化学法和红外等手段对修饰前后的环芬化合物进行了表征。循环伏安性质表明该修饰电极在pH<9的磷酸盐缓冲溶液中可稳定存在,且在0.1mol.L-1磷酸缓冲溶液(pH=6.80)中,-0.25^+0.80 V(vs.SCE)电位区间呈现两对氧化还原峰,其中第一对峰的式量电位EΘ’为+0.300 V(vs.SCE),且EΘ’随pH值增加而负移,并算得该对峰的电子转移数和参与电极反应的质子数均为1。该修饰电极对NADH的氧化具有良好的催化作用,降低氧化过电位超过300 mV;并求得在NADH存在下,电极扩散系数为1.4×10-6cm2.s-1,推测了NADH可能的电催化机理。NADH浓度在0.05~2.0 mmol.L-1范围内与峰电流呈现良好的线性关系,可在此浓度范围内进行定量分析。
The preparation of amide - based cyclophane graphite modified electrode and its electrochemical properties were studied. The modified electrode gives aquasi - reversible redox peaks in 0.1 mol/L phosphate buffer solution（ pH = 6.80 ） over the potential range from - 0.25 to 0.8 V （ vs. SCE ） , in which the formal potential of the first redox peak is 0. 300 V （ vs. SCE）. The formal potential shifts in negative direction with the increasing of pH values. The electrode reaction contains one electron and one proton. The amide - based cyclophane graphite modified,electrode shows an electrocatalytic activity for the oxidation of NADH in 0.1 mol · L^-1 phosphate buffer solution. The diffusion coefficienl of NADH was calculated as 1.4 × 10^-6 cm^2·s^-1 . The mechanism of the electrocatalytic oxidation of NADH was brought out. The peak currents were proportional to the Concentrations of NADH in the range of 0.05 - 2.0 mmol · L^-1 .
出处
《济南大学学报（自然科学版）》
CAS
2008年第1期51-55,共5页
Journal of University of Jinan(Science and Technology)
基金
济南大学科研基金(Y0412)
