摘要
根据表型性状选取少量辽宁绒山羊个体,直接进行类胰岛素生长因子-Ⅰ(IGF-Ⅰ)基因5′调控区克隆测序以确定单核苷酸多态(SNP)位点,共发现4个SNPs,分别是G→C(388bp)、A→G(668bp)、A→C(719bp)、G→A(752bp)的突变,导致5′调控区305～800bp中比野生型个体减少一个CdxA转录因子结合位点,但C/EBP的值(89.2)高于野生型(88.5).然后通过引入错配碱基创造酶切位点技术和多聚酶链反应-限制性片段长度多态性(PCR-RFLP)方法,对520只辽宁绒山羊进行基因型检测,结果表明,每个SNP位点在本群体中都有AA(野生型)、AB和BB(突变型)三种基因型,且4个SNPs位点共有13种单倍型组合.将不同SNP的基因型及单倍型组合与绒产量、绒纤维细度和绒纤维长度进行关联分析发现,SNP2位点的AA基因型绒纤维细度极显著低于AB型和BB型(P<0.01),而SNP4位点AA基因型产绒量显著高于AB型和BB型(P<0.05),单倍型组合H7H7与产绒量和绒纤维细度均有显著相关(P<0.05).IGF-Ⅰ基因可能是影响绒山羊产绒性状的主要候选基因.
Insulin-like growth factorⅠ（IGF-Ⅰ） is essential for the growth and differentiation of hair follicles which is an important part of wool and cashmere. But there is no report on polymorphisms of the IGF-Ⅰ gene in cashmere goat, and also few candidate genes for cashmere production traits have been reported in cashmere goats. The objectives of this work were to detect the single nucleotide polymorphism （SNP） in the 5′ flanking region of IGF-Ⅰ gene and to determine their association with fibre traits in Liaoning cashmere goats. The fibre traits data investigated in the experiment were combed cashmere weight, cashmere fibre length and cashmere fibre diameter. A few individuals of the Liaoning cashmere goats, selected according to phenotypic character, were used for SNPs detection in the 5′flanking region of IGF-Ⅰ gene, and four point mutation G→C （388 bp）, A→G （668 bp）, A→ C （719 bp）, G→A （752 bp） were identified, which result in a CdxA bonding-site lack, and score increase of C/EBP site in the 5′ flanking region compared with the wild type. Then different genotypes were detected in 520 Liaoning cashmere goats using create restriction site （CRS） and polymerase chain reaction-restriction fragment length polymorphism （PCR-RFLP）, three genotypes, AA, AB and BB were observed coded for by two different alleles A and B for each SNP, and 13 diplotypes were identified in the groups of the four SNPs. The relationships between the genotypes and combed cashmere weight, cashmere fibre length and cashmere fibre diameter were analyzed. The statistical analysis showed that animals with the genotype AA of SNP2 in Liaoning cashmere goats had the thinnest cashmere fiber diameter compared with those with the AB and BB genotype （P 〈 0.01）, and individuals with genotype AA of the SNP4 had the highest cashmere weight compared with those with the other genotypes （P 〈 0.05）. The relationship between diplotype and cashmere fiber traits was also analyzed, the results showed tha
出处
《生物化学与生物物理进展》
SCIE
CAS
CSCD
北大核心
2009年第5期566-573,共8页
Progress In Biochemistry and Biophysics
基金
国家科技支撑计划资助项目(2006BAD01A11)
国家高技术研究发展计划(863)资助项目(2007AA10Z151)~~
