摘要
采用单孢分离的方法分离培养金针菇粉孢子,对粉孢子的产生、萌发、核相及极性进行了研究。结果表明:单核菌丝和双核菌丝都产生粉孢子,粉孢子多呈圆柱或卵圆形,少数呈圆形或Y形。粉孢子很容易萌发,其芽管直径一般较粉孢子宽。单核菌丝产生单核的粉孢子,其极性与亲本菌丝相同;双核菌丝产生的粉孢子也为单核,未观察到双核或多核的粉孢子。双核菌丝产生的粉孢子一部分与组成双核体的一个亲本单核菌丝的交配型相同,另一部分与组成双核体的另一亲本单核菌丝有相同的交配型。
Single oidial isolates of Flammulina velutipes were obtained by isolatingoidia emerged from myceliujn. Their occurrence, germination, nuclear behavior andPOlarihes of sexuality of oidia in different oidial isolates were also detendned.The experimental resultS indicated that both the mono--and di--caryons could giverise to oidia. Most of oidia were found to be cylindrical or oval in shape, only a fewwere round or y--shaped. Oidia gewhnared easily and the diameter of germ tuble wasusually wider than that of oidia. india produced by monocaryons were all uninucleatoand had the same sexUality as that of the parental mycelium. Oidia from dictionswere also uninucleate. Binucleate or mulhnucleare oidia had not been discovered. APOrtion of the oidia produced by dicryon had the same mating type of one parentalmyceliuxn, while the other portion had the same mahng type as the other parentwhich gave rise tO the dicaryon.
出处
《菌物系统》
CSCD
北大核心
1998年第1期75-82,共8页
Mycosystema
关键词
金针菇
粉孢子
萌发
细胞核
极性
Flammulina velutipes
Oidia
Germination
Cell nucleus
Polarity ofsexuality