摘要
用增加膳食维生素Ｅ（ＶＥ）观察对冷应激大鼠红细胞（ＲＢＣ）膜丙二醛（ＭＤＡ）含量和钠，钾－ＡＴＰ酶［（Ｎａ，Ｋ）－ＡＴＰａｓｅ］活性的影响。结果表明：增加膳食ＶＥ摄入可以降低大鼠ＲＢＣ膜ＭＤＡ含量；冷暴露２４ｈ后，高膳食ＶＥ摄入组ＲＢＣ膜ＭＤＡ含量明显低于室温高膳食ＶＥ组；冷应激可使大鼠ＲＢＣ膜（Ｎａ，Ｋ）－ＡＴＰａｓｅ活性明显升高；冷应激时高膳食ＶＥ摄入，大鼠ＲＢＣ膜（Ｎａ，Ｋ）－ＡＴＰａｓｅ活性较低膳食ＶＥ大鼠升高更显著。相关分析显示，大鼠ＲＢＣ膜ＭＤＡ含量与（Ｎａ，Ｋ）－ＡＴＰａｓｅ活性间呈负相关。由此可见，增加ＶＥ摄入可以降低冷应激大鼠ＲＢＣ膜ＭＤＡ含量，增加膜（Ｎａ，Ｋ）－ＡＴＰａｓｅ活性，ＭＤＡ含量和（Ｎａ，Ｋ）－ＡＴＰａｓｅ活性间呈负相关，推测膳食ＶＥ可能通过抗体内脂质过氧化，清除自由基，保护细胞膜引起了细胞膜（Ｎａ，Ｋ）－ＡＴＰａｓｅ活性升高。
For elucidation of the effect of dietary Vitamin E to coldstress rat,we investigated the level of LPO product(MDA)and activity of (Na,K)ATPase in erythrocytic(RBC)membrane.40 adult male SpragueDawley rats were at random divided into 4 groups:high dietary VE(230μg/g) and coldesposed,low dietary VE(30μg/g) and coldexposed,high dietary VE and not coldexposed,low dietary and not coldexposed.The rats were fed at 18℃ for 2 weeks and later kept the Coldexpoised rats at 1±1℃ for 24h.The results showed that the levels of MDA in high dietary VE rats were obviously lower than those of low dietary VE rats(P<0 01),at the same time the activity of (Na,K)ATPase (P<0 01) no matter what diet was provided;and both high dietary VE and coldstress could increase the (Na,K)ATPase and the level of MDA in RBC membrane.The conclusion based on our findings was that either coldstress or dietary VE could increase the rat RBC membrane (Na,K)ATPase activities and VE could decrease the level of MDA in RBC membrane.We suggested that the change of the activity of ATPase was caused by the antioxidation effect of Vitamin E.
