摘要
为了研究粒－巨噬细胞集落刺激因子（ＧＭ－ＣＳＦ）对化疗药物诱导ＨＬ－６０细胞凋亡的影响，运用吖啶橙（ＡＯ）／溴乙锭（ＥＢ）染色法和ＤＮＡ电泳法检测了ＧＭ－ＣＳＦ对阿糖胞苷（Ａｒａ－Ｃ）、高三尖杉酯碱（ＨＨＴ）诱导ＨＬ－６０细胞凋亡的影响。结果提示同步作用时对Ａｒａ－Ｃ／ＨＨＴ诱导的细胞凋亡无影响，但在非同步时（前或后）对Ａｒａ－Ｃ／ＨＨＴ诱导的ＨＬ－６０细胞凋亡有抑制作用。
Using acridine orange (AO)/ethidium bromide (EB) staining and DNA electrophoresis, the effect of GMCSF on the HL60 cell line apoptosis induced by cytosine arabinoside (AraC)/homoharringtonine (HHT) was observed. GMCSF, combined with AraC/HHT at the same time, had no influence on HL60 cells apoptosis induced by AraC/HHT. However, when GMCSF was applied to the HL60 cells before or after AraC/HHT challenge, it inhibited the HL60 cells apoptosis induced by AraC/HHT. The results suggested that the optimal time of GMCSF application must be carefully determined in chemotherapy.
出处
《南京医科大学学报（自然科学版）》
CSCD
1998年第2期98-100,共3页
Journal of Nanjing Medical University(Natural Sciences)
