摘要
目的:探讨葡萄糖-6-磷酸-异构酶抗原(GPI抗原)及类风湿因子(RF)的检测对老年人类风湿性关节炎(EORA)的诊断价值。方法:分别用ELISA法、速率散射比浊法检测49例RA患者和49例非RA的老年健康对照者血清GPI抗原和RF。运用四格表和受试者工作特征曲线评估两者对EORA诊断效能。结果:GPI抗原对EORA的敏感性、特异性和准确性分别为81.63%、93.87%及87.75%,阳性和阴性拟然比分别为13.3和0.19。RF诊断EORA的敏感性、特异性和准确性分别为73.46%、79.59%、76.53%,阳性和阴性拟然比分别为3.16和0.35。GPI抗原诊断准确性高于RF(P=0.013)。GPI抗原诊断EORA的ROC曲线下面积(AUC)为0.913(95%CI,0.852-0.975),优于RF(P<0.001)结论:GPI抗原对EORA具有较好的敏感性和很高的特异性,在诊断EORA时较RF有更高的价值。
Objective： To evaluate the clinical diagnostic value of glucose-6-phosphate isomerase （GPI） in patients with onset rheumatoid arthritis （EORA）. Methods： GPI was detected by quantitative ELISA and rheumatoid factor by BNP nephelometer in 49 cases of EORA and 49 health control cases. The accuracy of GPI and RF examination was compared. Receiver operating characteristic （ROC） curves were used to assess the diagnostic value of GPI. Results： The sensitivity, specificity and accuracy of the GPI diagnostic method were 81.63 %, 93.87 % and 87.75% respectively, while those of RF were 73.46 %, 79.59 % and 76.53 % respectively. The positive and negative-likelihood ratio for the GPI diagnostic method was 13.3 and 0.19, while the values were 3.6 and 0.33 respectively for RF. Area under ROC in GPI examination was 0.913 （95%CI, 0.852-0.975）. Conclusions： GPI examination is accurate in diagnosis of EORA.
出处
《现代生物医学进展》
CAS
2009年第9期1726-1727,1730,共3页
Progress in Modern Biomedicine
