摘要
建立了HPLC法测定d-生物素的有关物质。采用C_(18)色谱柱,流动相为0.05%三氟乙酸溶液-乙腈(75:25),检测波长210 nm。d-生物素在10～1000μg/ml浓度范围内线性关系良好,检测限为0.5ng。有关物质的检测限均小于1.0 ng。
An HPLC method was established for the determination of related substances of d-biotin. A Ct8 column was used with the mobile phase of 0.05 % trifluoroacetic acid solution-acetonitrile （75 ： 25） at the detection wavelength of 210 nm. The calibration curve of d-biotin was linear in the concentration range of 10 - 1 000 μg/ml. The limit detection of d-biotin was 0.5 ng, as its related substances were all less than 1.0 ng.
出处
《中国医药工业杂志》
CAS
CSCD
北大核心
2009年第5期368-370,共3页
Chinese Journal of Pharmaceuticals
基金
国家科技兴贸行动专项(2006GH553302)
浙江省重大科技专项(2006C11190)资助
