摘要
用含不同浓度硒的Zarrouk培养液培养螺旋藻，用含不同浓度硒的豆芽汁葡萄糖培养酵母、并用含不同浓度硒的棉子皮培养基培养平菇、试验结果表明：在适宜的培养条件下，螺旋藻、酵母及平菇生长最适宜硬浓度分别为60mg·L(-1)，10mg·L(-1)和200mg·L(-1)，硒富集系数分别为19．2，14．4和22．6。将3种植物的蛋白质提出，经柱层析及电泳分离，分别得到多种含硒蛋白。试验证明。3种植物吸收的硒绝大部分都转化为含硒蛋白。
The optimal concentration of Na2SeO3 respectively for spirillum, yeast and mushroom cultivation was 60 mg·L(-1), 10 mg·Lu(-1) and 200 mg·L(-1). The eutrophication coefficient of selenium was 19. 2, 14. 4 and 22. 6. Selenoproteins in spirillum, yeast and mushroom. When three kind of protein were frationated using polyacrylamide gel electrophoresis and sephadex G-150 gel chromatography, they were purified. The result shows the majority of celenium that was absorbed by three microbes can be tranformed to the celenium-content protein.
出处
《中国农业大学学报》
CAS
CSCD
北大核心
1998年第1期11-15,共5页
Journal of China Agricultural University
