摘要
５只ＨＶＴ免疫鸡，实验感染ＭＤＶ后的第１～１０周期间，分别采用初次细胞分离培养物上清中的自由病毒粒子鉴定，ｄｏｔ－ｂｌｏｔＤＮＡ杂交和常规ＡＧＰ试验等方法，对并发感染鸡羽毛根进行了病毒及病毒抗原的动态检测与比较。结果显示，在上述羽毛根中可同时存在ＨＶＴ粒子和ＭＤＶＤＮＡ。ＨＶＴ可分离时间为ＭＤＶ攻毒后１～７周；ＭＤＶＤＮＡ最早检出时间为ＭＤＶ攻毒后的第１８～２２天，并达到其持续性（２２～７０ｄ）的检出率最高峰（１００％）；而ＡＧＰ法对此期间鸡只羽毛根ＭＤＶ抗原的最早检出时间在第２２天，且检出率很低。因此，羽毛病毒抗原ＡＧＰ检测法，对免疫鸡只或鸡群ＭＤＶ感染的诊断仍然是特异的，但较单纯感染的检测，其检出率更低，因而更难以反映出免疫鸡群中ＭＤＶ感染的真实状况。
Five chickens vaccinated with turkey herpesvirus(HVT)and challenged with virulent Mareks disease virus(MDV)at 14 days after vaccination During 1 to 10 weeks after chal lenge ,the HVT and MDV in the feather tips were detected by both identification of infectious cell free virus from culture medium of primary isolation and hybridization of a specific probe for MDV 1,while the viral antigens in the feather tips were assessed by AGP The concurrent viral maturation of both HVT and MDV were recovered in the feathers:The positive for HVT was recovered at 1 to 7 weeks post challenge by isolation The positive for MDV was identified by hybirdization at 18 days post challenge in some chickens and was always kept from 22 to 70 days post challenge MDV antigens activity of conventional AGP was found earlier at 22 days post Challenge,but it was found only infrequently and at lower levels in the feathers of vaccinated challenged chickens Thus,immunization of HVT did not affect diagnostic specificity of MDV infected chickens,but only droped greatly positive rates of AGP for the viral antigens in the feather tips of MDV infected chickens and made the AGP diagnosis even more difficult to reflect reality of MDV infection of the chickens vaccinated with HVT
出处
《畜牧兽医学报》
CAS
CSCD
北大核心
1998年第3期274-279,共6页
ACTA VETERINARIA ET ZOOTECHNICA SINICA
