摘要
为了探索酸雨对大豆种子萌发时能量代谢的影响,采用pH值分别为2.5,4.0的模拟酸雨及室内培养方法,测定不同酸雨胁迫强度对种子的ATP含量、能荷、过氧化氢酶活性、线粒体活性及呼吸速率的影响。结果表明:随着酸雨胁迫时间的延长,种子萌发ATP含量、能荷含量及过氧化氢酶活性呈先升后降的趋势,线粒体活性呈先升后降再平稳的趋势,呼吸速率呈现降-升-降-升的变化趋势。随着酸雨胁迫强度逐渐减弱,种子的ATP含量、能荷含量、过氧化氢酶活性及线粒体活性均逐渐升高。酸雨伤害增强,大豆种子呼吸作用与能量代谢减弱,酸雨胁迫强度与大豆萌发种子各呼吸指标间呈现剂量-效应关系。
In order to explore the effect of acid rain on energy metabolism of soybean seed germination, simulated acid rain of pH value 2.5,4.0 and greenhouses culture method were adopted, and energy metabolism indexes of ATP content, energy charge, CAT activity, mitochondrial activity and respiration rate were determined. ATP content , and CAT activity showed increase and then decrease trend. Mitoehondrial activity showed an increase till the3nd day, a decrease, and then remain stable. Respiration rate appeared down-up-down-up trend with the prolonged acid rain stress. ATP content, energy charge , CAT activity and mitoehondria activity were increased with the aggravation of acid rain stress. With the harm of acid rain increased ,the respiration and energy metabolism in soybean seed decreased,and acid rain stress intensity and respiratory index of germinating soybean seed showed a dose- response relationship.
出处
《大豆科学》
CAS
CSCD
北大核心
2009年第2期243-246,共4页
Soybean Science
基金
江南大学质量工程建设资助项目(20081218)
江苏省教育厅"高等学校大学生实践创新训练"(20070730)资助项目
关键词
酸雨
种子萌发
能量代谢
Acid rain
Seed germination
Energy metabolism
