摘要
利用含有筛选标记基因和目的基因Δ6-脂肪酸脱氢酶基因的双T-DNA共转化表达载体pLIN61,经农杆菌EHA101介导,采用子叶节转化法转化大豆,使用除草剂Glufosinate作为筛选剂,获得了一批携带有玻璃苣Δ6-脂肪酸脱氢酶基因的转基因大豆,转化效率为2.0%,共转化频率及实际转化率分别为56.25%、1.12%。PCR检测和Southern杂交结果证明,玻璃苣D6D已经整合到大豆的基因组上。RT-PCR检测结果显示,玻璃苣Δ6-脂肪酸脱氢酶基因在转基因大豆的转录水平上得到了表达。
The co-transformation expression vector, pLIN61 carrying two separate T-DNAs with selectable marker gene and △^6-desaturase gene was used to transformed soybean cotyledonary nodes mediated by Agrobacteriurn turnefaciens strain EHA101. A batch of transgenic soybeans harboring △^6-desaturase gene were obtained through Glufosinate screening. Research results demonstrate that the transformation efficiency, co-transformation frequency and real transformation frequency wrer 2.0%, 56.25% and 1.12% respectively. PCR detection and Southern analysis confirm that borage △^6-desaturase gene Gas integrated into soybean genome. RT-PCR detection showed that borage △^6-desaturase gene had expressed at transcriptional level in the transgenic soybeans.
出处
《大豆科学》
CAS
CSCD
北大核心
2005年第4期291-295,共5页
Soybean Science
基金
国家自然科学基金项目(30371000)
广东省自然科学基金项目(032239)
广东省科技攻关项目(2005B210201009)
华南农业大学校长基金项目(5100-K03003)
