摘要
目的了解监测结肠癌患者外周血癌胚抗原mRNA(CEA mRNA)和端粒酶活性(TA)表达的临床意义。方法应用逆转录-套式-聚合酶链反应技术检测CEA mRNA,比较结肠癌和结肠炎患者治疗前血液中表达CEA mRNA的差别以及结肠癌患者治疗后指标阳性者与阴性者36个月生存情况。结果结肠癌患者血液CEA mRNA阳性表达率显著高于结肠炎患者(P<0.01);结肠癌患者治疗后指标阴性组的36个月生存例数显著多于阳性组(P<0.01)。结论监测血液CEA mRNA有助于发现癌细胞血液转移和评估结肠癌患者的预后。
Objective To investigate the clinical significance of the expression of carcinoembryonic antigen mRNA （CEA mRNA） in peripheral blood of patients with colorectal cancer. Methods CEA mRNA in blood was detected by reverse transcriptase-nested primers-polymerase chain reaction. The difference of the expression of CEA mRNA in peripheral blood between colorectal cancer and colonitis were compared, and the survival condition between positive CEA mRNA and negative CEA mRNA in the patients after treatment was observed. Results The positive expression rate of CEA mRNA in blood in patients with colorectal cancer was significantly higher than that of patients with colonitis （ P 〈 0.01）. In the patients with colorectal cancer, after treatment the survival cases of 36 months in negative CEA mRNA group were much more than those of positive CEA mRNA group（ P 〈 0.01 ）. Conclusion The CEA mRNA in blood is a useful index in the discovery of haemal metastasis of tumor cells and the evaluation of prognosis for patients with colorectal cancer.
出处
《河北医药》
CAS
2009年第7期796-797,共2页
Hebei Medical Journal
基金
承德市科学技术研究与发展计划项目(编号:200521017)
河北省科学技术研究与发展计划项目(编号:07276101D-2)
关键词
结肠癌
血液
癌胚抗原MRNA
colorectal cancer
blood
carcinoembryonic antigen mRNA
