摘要
目的探讨噬菌体生物扩增法(PhaB)快速测定结核分枝杆菌对异烟肼(INH)和利福平(RFP)的药物敏感性在临床应用价值。方法选择初治肺结核病患者留痰后培养获得的结核分枝杆菌菌株69株和复治患者留痰后培养获得的结核分枝杆菌菌株98株。均经改良罗氏培养基培养并进行菌型鉴定,证实为结核分枝杆菌(MTB)。分别采用PhaB法和改良罗氏比例法检测其对INH和RFP的耐药性,将2种方法的结果进行敏感度、特异度和符合率比较。结果(1)初治结核分枝杆菌69株中,与改良罗氏比例法比较,PhaB法检测INH的敏感度、特异度和符合率分别为80%(4/5)、90.6%(58/64)和89.9%(62/69);PhaB法检测RFP的敏感度、特异度和符合率分别为100%(3/3)、95.5%(63/66)和95.7%(66/69)。(2)复治结核分枝杆菌98株中,与改良罗氏比例法比较,PhaB法检测INH的敏感度、特异度和符合率分别为89.7%(35/39)、88.1%(52/59)和88.7%(87/98);PhaB法检测RFP的敏感度、特异度和符合率分别为97.1%(34/35)、93.7%(59/63)和94.9%(93/98)。结论噬菌体生物扩增法同改良罗氏比例法比较有较高的敏感度、特异度和符合率,可快速、准确地检测结核分枝杆菌对INH和RFP的耐药性。
Objective To evaluate the clinical value at the primary level laboratories of phage amplified biologically assay （PhaB） in the detection of isoniazid and rifampin resistance in Mycobacterium tuberculosis. Methods Sixty-nine strains of Mycobacterium tuberculosis strains were obtained from the sputum of the primary pulmonaty tuberculosis cases. Ninety-eight strains were obtained from the sputum of the repeated pulmonary tuberculosis cases. The total 167 strains were cultured on Lo-wenstein-Jensen culturemedium and identified as Mycobacterium tuberculosis isolates. Iseniazid and rifampin resistance in Mycobactcrium tuberculosis were detected by the PhaB assay and the conventional proportion method, and the results of both methods were compared to each other in sensitivity, specificity and accuracy. Results （ 1 ） In 69 Mycobacterium tuberculosis isolates from primary pulmonary tuberculosis cases, in comparison with the results of the conventional proportion method, the sensitivity, specificity and accuracy of the PhaB assay on isoniazid were 80% （4/5）, 90.6% （58/64） and 89.9% （62/69）, respectively. In comparison with the results of the conventional proportion method, the sensitivity, specificity and accuracy of the PhaB assay on rifampin were 100% （3/3）, 95.5% （63/66） and 95.7% （66/69）, respectively. （2）Compared with the results of the conventional proportion method, the sensitivity, specificity and accuracy of the PhaB assay on isoniazid were 89.7 % （35/39）, 88.1% （52/59） and 88.7 % （87/98）, respectively. In comparison with the results of the conventional proportion method, the sensitivity, specificity and accuracy of the PhaB assay on rifampin were 97.1% （34/35）, 93.7% （59/63） and 94.9% （93/98）, respectively. Conclusion The PhaB assay has much higher sensitivity, specificity and accuracy in drug susceptibility test than the conventional proportion method. The PhaB assay is a rapid and accurate method in the detection of isoniazid and rifampin resistance i
出处
《河北医药》
CAS
2009年第7期789-792,共4页
Hebei Medical Journal
关键词
结核分枝杆菌
异烟肼
利福平
耐药性
噬菌体生物扩增法
改良罗氏比例法
Mycobacterium tuberculosis
isoniazid
rifampicin
resistance
phage amplified biologically assay
Lo-wenstein-Jensen culture medium
