摘要
埃博霉素是由黏细菌纤维堆囊菌产生的次级代谢产物。与紫杉醇相似,它们可以抑制微管解聚,使细胞有丝分裂终止在G2-M期。在P-糖蛋白表达型的多药耐药性肿瘤细胞系中维持很大的毒性,而且比紫杉醇有更好的水溶性。全合成埃博霉素虽然可以实现,但是与发酵方法相比尚无经济可行性。本文介绍了埃博霉素生物合成基因簇的克隆和异源表达,同时也对Red/ET重组技术的特点以及在埃博霉素生物合成中的应用进行了详细阐述。
Epothilones are secondary metabolites that are naturally produced by the myxobacterium Sorangium cellulosum, and like paclitaxel （Taxol）, they inhibit microtubule depolymerisation and arrest the cell cycle at the G2-M phase. They are effective against P-glycoprotein-expressing multiple-drug-resistant tumor cell lines and are more water soluble than paclitaxel. The total sythesis of epothilones has been achieved, but has not provided an economically viable alternative compared with to fermentation. We set out to cloning and heterologous expression of the epothilone gene cluster. The characteristic and development of Red/ET recombination and its application in biosynthesis of epthilones were described in this article.
出处
《药物生物技术》
CAS
CSCD
2009年第2期175-181,共7页
Pharmaceutical Biotechnology
