摘要
为黑曲霉的实际应用提供依据。以黑曲霉(Aspergillus niger)CZ2为菌株,进行了液体发酵产纤维产素为酶酶4∶3时条,最间件佳为的氮优5天源化。为。黑K确N曲定O霉了3,所碳黑氮产曲比纤霉为维的素5最∶1酶佳,碳各培氮组养含分条量酶件为活:麸4力%皮分,最和别适玉为产米:羧酶芯甲p为H基最为纤佳5维.碳8,素源最酶,佳麸活产皮力酶∶为玉温米6度6.芯为41的3U0最/m℃佳l,,比滤最纸佳例分解酶活力为61.73U/ml。
The aim of the study was to provide foundation for the practical application of AspergiUus niger. The optimal culture conditions for the production of cellulases by being liquid fermentation use Aspergillus niger CZ2. Results indicate that the highest level of cellulase activity is induced in the compound carbon source of wheat bran and corncob, and their optimal ration is 4：3, Potassium nitrate was the best nitrogen source, and their optimal ration was 1：4, the optimal ratio of C：N was 5：1, and the optimal mass concentration was 4%, the optimal conditions of enzyme were pH 5.8, The optimal culture temperature was 30℃, the optimal time was 5 days. The activities of cellulose compositions reached the maximal values. In the optimal culture condition, the activities of CMcase and FPAase were 66.41 U/ml, and 61.73 U/ml respectively.
出处
《中国农学通报》
CSCD
北大核心
2009年第9期74-77,共4页
Chinese Agricultural Science Bulletin
关键词
黑曲霉
纤维素酶
发酵条件优化
Aspergillus niger, cellulase, optimal fermentation conditions
