摘要
目的研究腺苷预处理对大鼠缺血／再灌注心肌细胞凋亡和凋亡蛋白Bcl-2／Bax的影响。方法制备缺血／再灌注损伤（I／R）和腺苷预处理的大鼠模型，采用流式细胞仪检测心肌细胞凋亡率和凋亡蛋白Bcl-2和Bax。结果①缺血／再灌注组和腺苷组心肌细胞凋亡率均显著高于对照组（P〈0．01），而腺苷组凋亡率显著低于缺血／再灌注组（P〈0．01）。②缺血／再灌注组抗凋亡蛋白Bcl-2表达与对照组比较无明显差别，而腺苷组Bcl-2显著高于缺血／再灌注组和对照组（P〈0．01）；缺血／再灌注组和腺苷组促凋亡蛋白Bax表达显著高于对照组（P〈0．01），而腺苷组Bax低于缺血／再灌注组（P〈0．01）；缺血／再灌注组和腺苷组Bcl-2／Bax比值显著低于对照组（P〈0．01），而腺苷组Bcl-2／Bax比值显著高于缺血／再灌注组（P〈0．01）。结论腺苷预处理明显抑制大鼠缺血／再灌注后心肌细胞的凋亡，并使Bcl-2／Bax比值增加。
Objective To investigate the effect of adenosine preconditioning on cardiac myocyte apoptosis and expression of apoptotic protein Bcl - 2/Bax during myocardial ischemia/reperfusion （ I/R ） in rats. Methods A model of I/R injury （ I/R group ） and pretreatment with adenosine （ AP group ） was reproduced in rats. Apoptotic rate of cardiac myocyte and expression of Bcl -2 and Bax proteins in apoptosis myoeardiac cells were observed by flow cytometry （ FCM ）. Result ①Apoptotic rate of cardiac myocyte was high in I/R group and AP group compared with control group （ P 〈 0.01） , but it is lower in AP group than that in I/R group（ P 〈 0.01）. ②There was no difference of the expression of anti -apoptotic protein Bcl- 2 btween I/R group and control group , but but it is higher in AP group than that in I/R group and control group （P 〈0.01 ）. The expression of proapoptotic protein Bax was high in I/R group and AP group compared with control group （P 〈 0.01 ） , but it is lower in AP group than that in L/R group （P 〈 0.01 ）. The Bcl- 2/Bax ratio was significandy lower in I/R group and AP group than that in control group （ P 〈 0.01 ）, but it is higher in AP group than that in I/R group（ P 〈 0.01 ）. Conclusions Adenosine preconditioning could significantly inhibite apoptosis of I/R cardiac myocyte and increase the Bcl - 2/ Bax ratio.
出处
《中国急救医学》
CAS
CSCD
北大核心
2009年第4期340-342,共3页
Chinese Journal of Critical Care Medicine
基金
基金项目：河北省科技厅科研基金资助项目（No.06276102D-100）
