摘要
目的观察行自体干细胞移植的淋巴瘤患者输注冷冻自体血小板的效果。方法选择6例行干细胞移植患者,移植前使用血细胞分离机采集患者自体血小板13次。在血小板中加入终浓度5%DMSO,于-80℃保存。当患者血小板小于20×109/L时,于37℃水浴箱快速解冻复苏后输注。分别测定冷冻前后血小板回收率以及22℃保存5d血小板的CD42b、CD62p及输注后1h血小板校正增加值(CCI)。结果血小板复苏后回收率为58%～85%,平均回收率为72.5%±8.2%。血小板CD42b由冷冻前的93.2%±6.0%下降到复苏后的80.1%±4.6%,CD62p由冷冻前的1.9%±0.9%上升到18.1%±10.1%(P<0.05),但与22℃保存5d的血小板CD42b、CD62p比较,差异均无统计学意义(P>0.05),在可接受的范围内。13例次的血小板输注中,11次1hCCI>7.5。结论造血干细胞移植患者输注自体血小板是安全且可行的,可替代异体血小板输注,有助于节约血源、避免输血传播疾病及减少输血引起的同种免疫。
Objective To explore the effect of autologous cryopreserved platelet,which were infused in lymphadenoma patients receiving autologous stem cells transplantation. Methods 13 harvest using COBE Spertra and infusion of 6 cases were studied. Platelet were cryopreserved in final concentration 5% dimethylsulfoxide,stored in -80℃ and thawed in a 37℃ aqueous bath, transfused when platelet counts dropped below 20 × 10^9/L. In vitro studies of fresh platelet,cryopreserved platelet and platelet stored 22℃ for 5 days,CD41,CD42b,CD62p,CD63 were performed. The recovery after freezing-thawing and a corrected count increment for 1 hour after transfusion were assayed. Results Platelet recovery after thawing was 58%-85% and the mean recovery was 72.5%±8.2%. The CD42b value of platelet decreased from 93.2% ±6.0% before cryopreserving to 80.1%±4.6% after thawing,the CD62p value increased from 1.9%±0. 9% to 18.1%±10. 1% （P〈0.05） after thawing. The CD42b,CD62p value of platelet between thawing and storing at 22℃ for 5 days was not significant different （P〈0. 05）. It indicated that is acceptable. The corrected count increment was above 7. 5 in ll of 13 infusion. Conclusions Autologous cryopreserved palatelet transfusion in autologous hemapoietic stem transplantation can prevent hemorrhage,obviating alloimmune and the transmission of virus. What＇s more,it was safe and practicable.
出处
《临床输血与检验》
CAS
2009年第2期123-126,共4页
Journal of Clinical Transfusion and Laboratory Medicine
关键词
血小板
冷冻保存
干细胞移植
自体输血
Platelet Cryopreserved Stem cells transplantation Autologous transfusion
