摘要
目的观察galectin-1对LoVo细胞生长的影响。方法构建galectin-1真核表达载体转染LoVo细胞,观察LoVo细胞生长情况。用免疫细胞化学方法检测转染后细胞galectin-1蛋白表达水平,免疫印迹检测转染后细胞Bcl-2表达的变化。结果成功构建了galectin-1真核表达载体pEGFP-C1/GAL1(p-GAL1),并建立了稳定的空载体(LoVo)细胞和真核表达载体转染细胞(p-LoVo细胞和p-GAL1-LoVo细胞)。p-GAL1细胞可表达galectin-1,而另2组细胞则不能。galectin-1表达可降低LoVo细胞Bcl-2的表达,诱导的细胞凋亡增加,3组细胞凋亡率分别为(9.61±0.56)%,(3.56±0.53)%和(3.46±0.46)%(P<0.001)。而细胞生长曲线表明,p-GAL1-LoVo细胞增殖与p-LoVo和LoVo细胞相似。结论galectin-1可促进LoVo细胞凋亡可能与galectin-1可降低LoVo细胞Bcl-2的表达有关。
Objective To observe the changes of LoVo cell growth by galectin-1. Methods Eukaryotic expression vector of galectin-1 was constructed and transfected into LoVo cells using lipofectamine^RM 2000. Immunochemistry was employed to detect galectin-1 expresson. LoVo cell proliferation and apoptosis were observed. Results Galectin-1 eukaryotic expression vector pEGFP-C 1/GAL1 was successfully constructed. Three cell clones, p-GAL1-LoVo and p-LoVo, transfected with pEGFP-CI/GAL1 and pEGFP-C1 correspondingly,and LoVo were cultured successfully. Galectin-1 expression downregulated Bcl-2 level only occurring in p-GAL1-LoVo cells, p-GAL1-LoVo cell proliferation was similar to p-LoVo and LoVo cells, but p-GAL1 -LoVo cell apoptosis was increased （ 9. 61 ±0. 56 ） % , as compared with p-LoVo and LoVo cells,[（3.56±0.53）% and （3.46±0.46）% respectively] （P〈0.001）. Conclusions Galectin-1 induces cell apoptosis possibly by downregulating Bcl-2 expresson.
出处
《中国普通外科杂志》
CAS
CSCD
北大核心
2009年第4期362-366,共5页
China Journal of General Surgery
