摘要
目的观察细胞膜galectin-1抗体阻断对LST-R1细胞与Ⅰ型胶原黏附的影响。方法激光共聚焦显微镜观察galectin-1在LST-R1细胞膜表面的表达。galectin-1抗体预孵LST-R1细胞后种植于Ⅰ型胶原(20μl/孔)包被的48孔板中,观察黏附细胞形态学差异;计数黏附细胞。结果galectin-1表达于LST-R1细胞膜表面,galectin-1抗体预孵LST-R1细胞后结合率为(38·2±0·92)%。ga-lectin-1抗体阻断组可见有渐多的不规则角形黏附细胞,且大部分细胞呈单个生长状态,而对照组大部分黏附细胞为圆形或类圆形,细胞主要呈聚集生长;黏附细胞数分别为40±4和30±4(P<0·01)。结论细胞膜表面galectin-1可能为LST病变呈浅表扩散的重要蛋白质分子之一。
Objective To observe the influence on adhesion between LST-R1 cell and collagen I mediated by galectin-1 antibody inhibition. Methods Laser confocal scanning microscopy was employed to detect the expression of galectin-1 on LST-R1 cell membrane, LST-R1 cells inhibited by galectin-1 antibody or not were seeded in the 48-well plate coated with collagen I （20μg/well）, and the morphology of the adhesive LST-R1 cells was observed and the adhesive cells were counted. Results Laser confocal scanning microscopy showed the expression of galectin-1 on the membrane of LST-R1 cells. The binding rate between galectin-1 and its antibody was 38. 2%±0. 92%. In the galectin-1 antibody inhibiting group,more and more irregular angular adhesive cells appeared, and most of the adhesive cells were growing in singles, while the adhesive cells,mainly clustered, in the control group were round or roudish. The numbers of adhesive LST-R1 cells in the antibody inhibiting group and the control group were 40 4 and 30 4 respectively （P〈0. 01）. Condusions Membrane galectin-1 may be involved in LST lesion which spread superficially.
出处
《解放军医学杂志》
CAS
CSCD
北大核心
2006年第3期210-212,共3页
Medical Journal of Chinese People's Liberation Army
