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一个二粒小麦LRR类受体蛋白激酶基因的克隆和鉴定 被引量:1

Cloning and characterization of a LRR-receptor-like protein kinase gene from Triti-cum turgidum
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摘要 Near the HMW-glutenin gene of wheat (Triticum aestivum), there is a locus (temporarily named TaXa) encoding LRR-receptor-like protein kinase, which is homologous to disease resistance protein Xa21 of rice (Oryza sativa) . Through RT-PCR approach, a cDNA clone of ZS2002 was isolated from the orthologous locus of TaXa in Triticum turgidum. ZS2002 was 3 081 bp long and encoding a peptide composed of 1 026 amino acid. The protein included N-terminal conserved sequence, LRR domains, a transmembrane region and a serine/threonine protein kinase domain. ZS2002 was expressed in root, stem, leaf and spike. The transcribing in seedling leaves was significantly enhanced by Blumeria graminis f.sp. tritici. TaXa gene might play a role in powdery mildew resistance reaction in Triticum. Near the HMW-glutenin gene of wheat (Triticum aestivum), there is a locus (temporarily named TaXa) encoding LRR-receptor-like protein kinase, which is homologous to disease resistance protein Xa21 of rice (Oryza sativa) . Through RT-PCR approach, a cDNA clone of ZS2002 was isolated from the orthologous locus of TaXa in Triticum turgidum. ZS2002 was 3 081 bp long and encoding a peptide composed of 1 026 amino acid. The protein included N-terminal conserved sequence, LRR domains, a transmembrane region and a serine/threonine protein kinase domain. ZS2002 was expressed in root, stem, leaf and spike. The transcribing in seedling leaves was significantly enhanced by Blumeria graminis f.sp. tritici. TaXa gene might play a role in powdery mildew resistance reaction in Triticum.
出处 《植物病理学报》 CAS CSCD 北大核心 2009年第2期217-220,共4页 Acta Phytopathologica Sinica
基金 河南省杰出人才创新基金(0621001700)
关键词 类受体蛋白激酶 蛋白激酶基因 二粒小麦 胞外结构域 鉴定 克隆 R类 信号传导途径 Triticum turgidum leucine-rich repeat (LRR) receptor-like protein kinase (RLK) cloning characterization
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