摘要
目的：建立高效液相色谱法测定人血浆中万古霉素的药物浓度方法。方法：采用液-液萃取法并选用去甲万古霉素为内标，以Symmetryshield RP18柱为色谱柱。流动相为甲醇：水（以磷酸二氢钾调节pH至3．1）（16：84），流速1．0mL·min^-1。Waters2487双波长紫外检测器，检测波长210nm。结果：线性范围为（0．63-63．0）mg·L^-1，r=0．9996（n=6）；平均回收率大于85％，低、中、高浓度样品的日间和日内RSD均小于5％。结论：本法适用于万古霉素的血药浓度测定。
OBJECTIVE To establish an HPLC method for determining plasma level of vancomycin. METHODS The new HPLC procedure involved a liquid-liquid extraction pretreatment procedure and an HPLC internal standard, norvancomycin. A symmetryshield RP18 column was used. The mobile phase of a mixture of methanol water （16：84,pH 3. 1） was used at a flow rate of 1.0 mL·min ^-1. The detector was Waters 2487 Dual λ Absorbance Detector, detector was set at the wavelength of 210 nm. RESULTS The calibration curve was linear in the range of 0. 63 to 63.0 mg· L^- 1. The average recovery was more than 85%, and the RSD within day and between-day were all less than 5%. CONCLUSION This method is suitable for monitoring of vancomycin in plasma.
出处
《中国医院药学杂志》
CAS
CSCD
北大核心
2009年第8期637-639,共3页
Chinese Journal of Hospital Pharmacy
关键词
高效液相色谱法
万古霉素
血药浓度
high performance liquid chromatography
vancomycin
plasm drug concentration
