摘要
对2005、2006年夏在南京农业大学江浦农场试验田发生的大豆根腐病,采用特异性PCR检测到发病组织中有大豆疫霉,经室内诱捕和分离,从发病田块的土壤和发病植株上共分离到4个大豆疫霉菌株PNJ1、PNJ2、PNJ3和PNJ4。用含有不同抗病基因的14个鉴别寄主测定这4个大豆疫霉菌株的毒力公式,PNJ1和PNJ2为1d,2,3b,3 c,4,6,7;PNJ3为1 a,1b,1 c,1d,1k,2,3b,3 c,5,7;PNJ4为1 a,1b,1 c,1d,1k,2,3b,3 c,4,6,与国际上已经报道的大豆疫霉菌株的毒力公式不同,为新的生理小种。该研究可为抗病品种的选育及利用提供科学依据。
Soybean root rot caused severely losses on Jiangpu Farm, Nanjing Agricultural University during the growing season of 2005 and 2006. Phytophthora sojae was detected by a rapid and specific PCR in the diseased tissues. Four isolates （ PNJ1, PNJ2, PNJ3 and PNJ4） were isolated from the diseased tissues of soybean and soil samples, and identified as Phytophthora sojae Kanufman ＆ Gerdemann. By using the method of hypocotyls inoculation on 14 differential hosts, PNJ1, PNJ2, PNJ3 and PNJ4 were identified as new races, and their virulence formula is 1d,2,3b,3c,4,6,7; 1d,2,3b,3c,4,6,7; 1a,1b,1c,1d,1k,2,3b,3c,5, 7 ; 1 a, 1 b, 1 c, 1 d, 1 k,2,3b,3c ,4,6, respectively. This research may provide useful information for breeding and using resistant varieties in Jiangsu area.
出处
《南京农业大学学报》
CAS
CSCD
北大核心
2009年第2期61-64,共4页
Journal of Nanjing Agricultural University
基金
公益性行业(农业)科研专项项目(nyhyzx07-053)
"长江学者和创新团队发展计划"创新团队项目
江苏省科学技术厅高技术项目(BG2006308)
关键词
大豆根腐病
大豆疫霉
生理小种
soybean root rot
Phytophthora sojae
physiological race
