摘要
目的：了解一氧化氮合酶（ＮＯＳ）在大鼠胃壁的分布规律。方法：采用ＮＡＤＰＨ－黄递酶（ＮＤＰ）组化法和整装铺片技术对ＮＯＳ在大鼠胃壁的分布进行定量和定位研究。结果：ＮＯＳ广泛分布于大鼠胃壁内，主要定位于肌间神经丛，在粘膜下丛、胃粘膜表面、胃腺及血管壁等处亦有分布。胃肌丛中ＮＯＳ阳性神经元形态基本类似，以圆形和卵圆形为主，但在胃不同区域神经元的分布密度、胞体大小和染色强度存在较大差异。密度分别为胃窦部：６２．１±３７．７个／ｍｍ２；胃体部：４３．４±３１．７个／ｍｍ２；胃底部：３１．６±２７．８个／ｍｍ２；胃底部神经元胞体较大，染色较深；胃窦部神经元大小不等，染色深浅不一；胃体部的则介于二者之间，呈过渡态。一些神经纤维的膨体呈ＮＯＳ阳性反应，它们串在一起形成“串珠”样结构，且多与血管和肌纤维伴行。结论：①一氧化氮（ＮＯ）可能参与对胃的运动、分泌和血液循环的调节。②ＮＯＳ阳性神经元在胃各部肌间神经丛中的分布差异可能与胃的生理功能密切相关。
Objective: To investigate the regularity of distribution of nitric oxide synthase (NOS) in the gastric wall in rats. Methods: The distribution of NOS in the gastric wall was quantitatively and localizationally determined with NADPHdiaphorase (NDP) histochemical staining and wholemount preparation. Results: NOS was widely distributed in the gastric wall and most of it was localized in the myenteric plexus. The shape of NOS positive neurons was basically similar (round or oval) while their density, size and staining intensity varied greatly in different parts of the stomach. The density was 62.1±37.7 cells/mm2, 43.4±31.7 cells/mm2 and 31.6±27.8 cells/mm2 respectively in the antrum, body and fundus. The size and staining intensity of NOS positive neurons in the fundus were resemble. However, they were different in the antrum. In the body, the characteristics of NOS positive neurons were at an intermediate state from the fundus to antrum. Beadlike structures were strung together by NOS positive varicosities in nerve fibers, some of which were closely adherent to the outer wall of blood vessels and smooth muscle fibers. Conclusion: ①Nitric oxide might be involved in the modulation of motility, secretion and blood circulation of the stomach; ②The significant difference of the distribution of NOS positive neurons among the myenteric plexus in different parts of the stomach may be related to the physiological function of the stomach.
出处
《第三军医大学学报》
CAS
CSCD
北大核心
1998年第2期124-127,共4页
Journal of Third Military Medical University
基金
全军"九五"攻关项目
关键词
一氧化氮合酶
胃
酶组织化学
大鼠
NDP
nitric oxide synthase (NOS)
NADPHdiaphorase (NDP)
stomach
enzyme histochemistry
rat
