摘要
观察重组溶葡萄球菌酶经口给药对大鼠的急性毒性和长期毒性。急性毒性试验中,大鼠以每千克体重5 000 mg的剂量口服重组溶葡萄球菌,观察中毒症状并测定LD50。长期毒性试验中,大鼠以每天每千克体重口服72,360,1 800 mg重组溶葡萄球菌酶,连续给药180 d,观察给药后45 d、90 d、135 d和180 d时大鼠的生长发育、血液学、血液生化学、组织病理变化。结果表明,重组溶葡萄球菌酶口服对大鼠的LD50大于5 000 mg/kg,长期毒性试验未见明显毒性反应。说明重组溶葡萄球菌酶在规定剂量下使用是安全可靠的。
The experiment was conducted to observe the acute toxicity of recombinant lysostaphin （rlysostaphin） and its long-period toxicity to rats. In the acute toxicity experiment, the rats were orally administrated rlysostaphin at a dosage of 5 000 mg/kg, and then the symptoms were observed and 50 percent lethal dose （ LD50 ） was calculated. In the long-period toxicity experiment, the rats were orally administrated rlysostaphin at different dosages of 72, 360, 180 mg/kg, and the administrations were lasted for 180 d. Body weight, hematological and hematobiochemical parameters, and histopathological changes were measured in rats at 45 d, 90 d, 135 d and 180 d after oral administration. The results showed that the LDs0 of rlysostaphin was over 5 000 mg/kg in rat. No significant toxic effects were found in the rats with a long-term oral administration. The results indicated that rlysostaphin is safe to use in accordance with the prescribed dose.
出处
《畜牧与兽医》
北大核心
2009年第4期24-27,共4页
Animal Husbandry & Veterinary Medicine
基金
十一五国家科技支撑计划项目(No.2006BDA31B00)
