摘要
目的:观察佐剂关节炎(adjuvant arthritis,AA)大鼠ApoA1、HDL和VEGF、E选择素及血管内皮超微结构的变化及新风胶囊(XFC)对其的影响。方法:用弗氏完全佐剂(Freund′scompleteadjuvant,CFA)诱发大鼠产生关节炎,观察AA大鼠ApoA1、HDL、VEGF、E选择素、细胞因子、关节肿胀度、关节炎指数(arthritisindex,AI)及血管内皮超微结构的变化以及XFC对其的影响,并进行相关性分析。结果:AA大鼠ApoA1、HDL差异率的变化:模型组ApoA1、HDL升高率明显高于正常组、XFC组、MTX组(P<0.05),与TPT组无显著差异(P>0.05)。XFC能下调AA大鼠ApoA1、HDL的表达(P<0.05)。XFC组与正常对照组无显著差异(P>0.05)。AA大鼠VEGF、E选择素及IL-1β、IL-10、CRP的变化:与模型对照组相比,正常组、XFC组VEGF、E选择素、IL-1β、CRP显著降低(P<0.01或P<0.05),IL-10显著升高;XFC能显著下调AA大鼠VEGF、E选择素、IL-1β、CRP的表达,上调IL-10的表达。AA大鼠血管超微结构的变化:与正常对照组相比:模型组、XFC、甲氨喋呤(MTX)、雷公藤多苷片(TPT)组血管内皮超微结构均出现不同程度破坏:与模型对照组相比:XFC、甲氨喋呤(MTX)、雷公藤多苷片(TPT)组血管内皮超微结构改善明显,XFC组改善明显优于TPT组、MTX组。XFC对AA大鼠足跖肿胀度、关节炎指数的影响:与模型对照组相比,XFC、甲氨喋呤(MTX)、雷公藤多苷片(TPT)组肿胀度均明显下降(P<0.05),与正常对照组相比无明显差异(P>0.05);与模型对照组相比,各组AA大鼠的关节炎指数均显著降低(P<0.05),XFC组与TPT组相比无明显差异(P<0.05)。AA大鼠ApoA1、HDL与足跖肿胀度、关节炎指数、VEGF、E选择素及细胞因子的相关分析:模型对照组ApoA1、HDL与VEGF、E选择素、IL-1β呈显著负相关(P<0.05或P<0.01),与足跖肿胀度、关节炎指数、CRP呈显著正相关(P<0.05或P<0.01),与IL-10无明显相关。结论:免疫应激引起AA大鼠ApoA1、HDL的改变,AA大鼠ApoA1、HDL的改变与疾
Objective： Observing changes of adjuvant arthritis （ adjuvant arthritis, AA） rats ApoA1, HDL and VEGF, Eselectin, BVE Ultramicro-structure, and Xinfeng Capsule （XFC） ＇s impact on Them. Methods：Using Freund＇s complete adjuvant（ Freund＇s complete adjuvant,CFA） to induce arthritis in rats,observing changes of AA rats ApoA1, HDL,VEGF, E-selection, cytokines ,joint swelling, arthritis index （ arthritis index, AI）, BVE Ultramicro - structure, and XFC＇s Impact on them, and doing correlated analysis. Results ： 1. Changes of AA rats ApoA1, HDL difference in rates ： Model group ApoA1 ,HDL＇s increased rate was significantly higher than that of the normal group,XFC group and MTX group（ P 〈 0. 05）, while it has no obvious difference with TPT group（P 〉 0. 05 ）. XFC can lower AA rats ApoA1, HDL expression（ P 〈 0.05 ）. XFC group has no obvious difference with normal group（ P 〉 0.05 ）. 2. Changes of AA rats VEGF,E-selection and IL - 1 β, IL - 10, CRP： Compared with the model group, the normal group, XFC group, E - selection, IL - 1β, CRP reduced significantly （ P 〈 0. 01 or P 〈 O. 05 ）, L - 10 increased obviously ; XFC can significantly lower AA rats VEGF, E -selection, IL- 1β,CRP expression, increase IL- 10 expression. 3. Changes of AA rats＇ BVE Uhramicro-structure： Compared with the normal group： BVE Ultramicro - structure of model group, XFC, MTX, and TPT group all have varying degrees of tissue damage;compared with the model group： BVE Ultramicro- structure of model group, XFC, MTX, and TPT group improves obviously. 4. XFC＇s impact on foot swelling rate and arthritis index of AA rats：Compared with model control group, swelling rate of XFC, MTX,TPT group decreased obviously （P 〈 0. 05）, while has no significant difference with normal control group（P 〉 0. 05 ） ;compared with model control group, AA rats arthritis rates in each group were significantly lower （P 〈 0. 05） ; XFC group has no obvious difference with TPT group （ P
出处
《中华中医药学刊》
CAS
2009年第4期734-737,共4页
Chinese Archives of Traditional Chinese Medicine
基金
国家中医药管理局科研课题专项基金(04-05LP27)
安徽省十一五攻关项目(07010300204)
安徽科技重点研究项目(06023068)
