摘要
目的:观察佐剂关节炎(AA)大鼠行为、脑组织氨基酸、大脑皮质神经细胞超微结构的变化及新风胶囊(XFC)对其的影响及可能的作用机制。方法:55只大鼠随机分为正常组(NC组),模型组(MC组),甲氨喋呤组(MTX组),雷公藤多苷片组(TPT组)及XFC组各11只。除正常组外,各组大鼠右后足跖皮内注射弗氏完全佐剂(FCA)0.1 ml制成AA模型,并于成功后的第19天开始,MTX组、TPT组及新风组分别给予相应的药物灌胃共30 d。结果:①与NC组比较,MC组大鼠行为改变自主活动次数减少,跳台训练期和测试期的错误次数增多,跳下平台的时间(SDL)缩短而反应时间(EL)延长(均P<0.01);脑组织中γ-氨基丁酸(GABA)含量升高,谷氨酸/γ-氨基丁酸(GLU/GABA)值降低(均P<0.05);神经细胞内线粒体肿胀、空泡样变,嵴突破坏,闰盘结构不完整,粗面内质网扩张。②与MC组比较,XFC组大鼠的自主活动次数增加、跳台错误次数减少、SDL延长、EL缩短(均P<0.05);GABA下调,GLU/GABA上调(均P<0.05),而MTX、TPT组改善不明显。XFC组、MTX组及TPT组大鼠神经细胞整体结构及大部分嵴突完整,个别线粒体嵴紊乱,粗面内质网无扩张。结论:AA大鼠可出现行为改变及脑组织氨基酸、神经细胞超微结构的变化,XFC能调节AA大鼠脑组织氨基酸、改善神经细胞超微结构和行为改变,这可能是其神经生物学及病理学的作用机制。
Objective： To observe the changes in behaviors, brain amino acids and neurocyte ultrastructures in rats with adjuvant arthritis （AA） and the effects of Xinfeng capsule （XFC）. Methods： Fifty-five rats were randomly divided into groups normal control（NC）, model control（MC）, methotrexate（MTX）, tripterygium wilfordii polycoride tablet （TPT） and XFC, 11 in each. Except for NC group, the remaining groups were intracutaneously injected with 0. 1 ml of Freunds complete adjuvant in the right hindlimb. The changes in behaviors, brain amino acids and neurocyte ultrastructure, and the effects of XFC were observed. Results： （1） Compared with NC group, the times of autonomic activities of MC group were obviously decreased, numbers of errors in exercise period and test period were obviously increased, step down latency （SDL） shortened and escape latency （EL） were lengthened. Gamma aminobutyric acid （GABA） levels were higher and glycine/GABA （GLU/GABA） was lower in the MC group than in the NC group （P〈0. 05）. Neurocyte mitochondria swelled, had vacuolar changes, intercalated disks structural integrity damaged, and rough endoplasmic reticulum （RER） expanded in MC group. （2） Compared with MC group, the times of autonomic activities in XFC group were obviously increased, number of errors in exercise period and test period was obviously decreased, SDL lengthened and EL shortened. GABA levels were lower, and GLU/GABA was higher in XFC group than in MC group （P〈0. 05）. Neurocyte overall structure integrity, the majority of cristae integrity, individual mitochondrial cristae disorder and no expansion of rough endoplasmic reticulum were found in XFC, MTD and TPT groups. Conclusion： AA rats have behavioral, brain amino acids and neurocyte ultrastructure changes. While improving their behaviors, XFC can offer an overall improvement in AA rats＇ brain amino acids and neurocyte ultrastructure, which may be the basis of neurobiology and pathology in the changes of their
出处
《中国康复》
2008年第4期219-222,共4页
Chinese Journal of Rehabilitation
基金
国家中医药管理局科研课题专项基金(04-05LP27)
安徽省十一五攻关项目(07010300204)
安徽科技重点研究项目(06023068)
