摘要
目的:研究中药复方有效部位(TCM-ES)的体外抗流感病毒FM1株(IFV-FM1)的作用。方法:采用微量细胞病变抑制法、中性红染色法、空斑抑制法,从TCM-ES的最大无毒浓度开始,按Reed-Muench法计算药物对FM1感染狗肾传代细胞(MDCK)的半数有效浓度(EC50),最小有效浓度(MTC),治疗指数(TI)。进行空斑计数(PFU),计算其半数抑制浓度(IC50)。结果:微量细胞病变抑制法表明,TCM-ES抗FM1的EC50为(300±18.3)mg/L,MTC为(75±6.8)mg/L,TI为(7.1±0.7);而利巴韦林的3项指标分别为(52.3±10.1)mg/L、(25±4.1)mg/L和(20.8±5.1)。中性红染色法显示,两药物的IC50分别为(285.0±19.2)mg/L和(45.3±4.9)mg/L,TI分别为(7.2±0.6)和(21.2±3.1)。两种方法测定的TCM-ES和利巴韦林的TI值无统计学差异(P>0.05)。通过PFU计数,TCM-ES的半数抑制浓度(IC50)为300mg/L,利巴韦林的IC50为50mg/L,与前述结果亦无统计学差异(P>0.05)。结论:TCM-ES体外具有一定的抗流感病毒作用,可作用于细胞内病毒,但其效果略差于利巴韦林,具有进一步研究和开发的价值。
Objective：To study the inhibitive effects of an effective section of a prescription of traditional Chinese medicine（ TCM- ES） on influenza virus A FM1 strain in vitro. Methods：The experiments were performed by microcytopathic-inhibiting-assay, Neutral Red stain and inhibiting plaque-forming units （PFU） test on MDCK cell strain. By means of observing the eytopathic effects （CPE）, measuring the absorbanee [ D（ A ）] and counting the PFU, according to Reed-Muench assay, the TCM-ES＇s effective dosage of 50 per-centage （ECso） and treatment index （TI） to FM1 were calculated. The inhibiting dose of 50 percentage of PFU （ICso） was also fig- ured up. Results：By CPE assay, TCM-ES＇S ECso, MTC and TI to 100TCIDs0 FM1 strain infection were （300 ± 18. 3 ）mg/L, （75 ± 6. 8 ） mg/L and （7. 1 ± 0. 7 ）, respectively; Whereas, ribavirin＇s ECso, MTC and TI was （ 52.3 ± 10. 1 ） rag/L, （25 ± 4. 1 ） mg/L and （20. 8 ± 5. 1 ）, respectively. By Neutral Red stain assay, TCM-ES＇ s ICso and TI was （ 285.0 ± 19. 2 ） mg/L and （7. 2 ± 0. 6 ）, respectively; Whereas ribavirin＇s IC50 and TI was（45.3 ± 4. 9 ）mg/L and （21.2 ± 3.1 ）, respectively. By reducing PFU assay,The ICso of TCM-ES and ribavirin was 300 mg/L and 50 mg/L, respectively. All the results above were almost consistent with each other（P 〉 0. 05）. Conelusions：TCM-ES assumes antiviral action on IFV-FMI strain in a certain degree in vitro and can rebel intracellular virus. But it is worse than the positive control medicine of ribavirin and is worthy of further study.
出处
《中药材》
CAS
CSCD
北大核心
2009年第3期391-394,共4页
Journal of Chinese Medicinal Materials
关键词
流行性感冒病毒
中药
有效部位
抗病毒作用
细胞病变效应
Influenza virus
Traditional Chinese medicine
Effective section
Antiviral effect
Cytopathic effect
