摘要
目的观察中药有效部位新药TCM-ES体内抗流感病毒的作用,以及体外抗菌效果,探讨其祛邪作用。方法采用鸡胚培养法,从TCM-ES的鸡胚最大无毒剂量(TC0)开始,预先注射不同剂量药物,再以100EID50(50%鸡胚感染量)流感病毒FM1株感染,收集尿囊液,测定血凝效价,能抑制32倍以上所注射的最小药量,即为最小抑制剂量(MIC)。以不同剂量的TCM-ES灌胃给药,以15LD50FM1株病毒滴鼻感染小鼠,测定小鼠肺指数和肺指数抑制率,死亡保护率,延长生命率等,观察TCM-ES的体内抗病毒作用。采用试管稀释法,测定TCM-ES的最低抑菌浓度(MIC)和最低杀菌浓度(MBC)。结果TCM-ES对鸡胚的TC0=2.5mg/胚,MIC=10mg/mL,对IFV的抑制作用比利巴韦林稍弱。动物实验显示,病毒对照组的肺指数和体重下降值明显升高,与正常对照组相比,具有非常显著性差异。TCM-ES在750mg/kg(中剂量)和1500mg/kg(大剂量)组可逆转小鼠体重的下降,肺指数显著高于感染对照组,与正常对照组比较,无显著性差异。TCM-ES中剂量组和大剂量组对流感病毒FM1株感染动物的平均存活日、死亡保护率、延长生命率与病毒对照组相比具有显著性差异。TCM-ES对金黄色葡萄球菌(G+)的MIC和MBC均为10mg/mL,而对革兰阴性杆菌无作用。结论TCM-ES体内具有抗流感病毒作用,对金黄色葡萄球菌有一定的抑制作用,具有进一步开发研究的价值。
Objective To study the antiviral and antibacterial effects of the effective site of traditional Chinese medicine （TCM- ES）. Methods Chicken embryoes were infected with influenza virus A （FM1 strain） and pre- injected with TCM - ES by means of chicken - embryo inoculation technique, and the antiviral effect of TCM - ES on chicken embryo was assayed by detecting the hemagglutination titers in allantoic fluids. Mice were orally pretreated with various dosages of drugs twice daily for 3 days, then were given drugs continuously for another 4 days following FM 1 infection. The protective effects of TCM -ES on mice infected with FM1 were assayed by calculating the weight, index of lung, death -protection rate, and life - prolongation rate, etc. Ribavirin was used as the positive control. In addition, the antibacterial effects of TCM -ES were observed by detecting the minimum inhibitory concentration （MIC） and minimum bactericidal concentra- tion （MBC） by test- tube dilution method. Results In chicken embryo experiments, TCM -ES showed a potential in- hibiting effect on influenza virus with the MIC of 10 mg/mL, which was weaker than ribavirin. The results of animal exper- iment showed that the body - weight （BW） and pulmonary index of infected model group decreased evidently compared with those of the normal group, TCM - ES groups at the dosages of 750 mg/kg and 1500 mg/kg could reverse the decrease of BW and lung index as compared with the infected models, the difference being insignificant as compared with the normal group. Moreover, TCM - ES also increased the death - protection rate and life - prolongation rate of mice in a dose - de- pendent manner. TCM -E5 at dosage of 10 mg/mL （MIC and MBC） had an antibacterial effect on staphylococcus, while had no effect on gram-negative bacilli. Conclusion TCM- ES has obvious antivirus effect on influenza virus FM1 strain, and also has certain antibacterial effect on staphylococcus, which is worth of further development and research.
出处
《中药新药与临床药理》
CAS
CSCD
北大核心
2009年第2期115-119,共5页
Traditional Chinese Drug Research and Clinical Pharmacology
