摘要
目的分析血管内皮生长因子（VEGF）和基质金属蛋白酶7（MMP-7）在大肠癌中的表达及临床意义。方法选取70例大肠癌患者癌组织、癌旁组织及正常组织标本，应用免疫组化方法检测VEGF、MMP-7表达和微血管密度（MVD）计数，并结合病理特点进行分析。结果VEGF、MMP-7表达阳性率大肠癌组织较癌旁组织、正常组织差异有统计学意义（VEGF：77．14％V85．71％，12．86％，P〈0．01；MMP-7：68．57％vs8．57％，5．71％，P〈0．01）；在大小为〈2cm、2～5cm和〉5cm肿瘤VEGF的阳性率分别为42．86％、92．86％和78．57％，MMP-7的阳性率分别为28．57％、75．00％和83．33％；分化程度高、中、低肿瘤VEGF的阳性率分别为71．43％、70．59％和90．91％，MMP-7的阳性率分别为71．43％、64．71％和72．73％，差异均有统计学意义（P〈0．05）。大肠癌VEGF、MMP-7阳性组与阴性组MVD平均值比较，差异均有统计学意义（t=3．23，P〈0．01；t=3．89，P〈0．01）；大肠癌有淋巴转移组与无淋巴转移组MVD平均值比较，差异有统计学意义（P〈0．01）。结论VEGF、MMP-7与大肠癌的高血管生成活性相关，VEGF、MMP-7有望成为抗大肠癌侵犯转移治疗的靶蛋白。
Objective To study the expression of vascular endothelial growth factor （VEGF） and matrix metalloproteinase-7 （MMP-7） in human colorectal cancer and validate the significance and distinction of angiogenesis in vascular invasion and metastasis. Methods VEGF and MMP-7 expression and microvessel density （MVD） were detected by immunohistochemistry in surgically resected specimens （cancer tissues, cancer-adjacent tissues and normal tissues） from 70 colorectal cancer patients, and the results were analyzed with clinical pathologic characteristics. Results VEGF and MMP-7 expression were higher in colorectal cancer tissues than those in the cancer-adjacent and controls （VEGF：77. 14% vs 5.71% , 12.86% , P 〈 0.01 ; MMP-7 ： 68.57% vs 8.57% , 5.71%, P 〈0.01 ）. The positive rates of VEGF and MMP-7 expression were related to the tumor size （ 〈 2 cm, 2 -5 cm and 〉5 cm in diameter, VEGF： 42.86%, 92.86% and 78.57% ; MMP-7： 28.57%, 75.00% and 83.33% ; all P 〈 0.05 ） and the tumor differentiation （ high, moderate and low differentiation, VEGF： 71.43%, 70.59% and 90.91%, MMP-7 ： 71.43%, 64.71% and 72.73% ; all P 〈 0.05 ）. VEGF and MMP-7 expression in metastasis-positive group were 100% and 95.24%, respectively. The mean values of MVD were significantly different between VEGF or MMP-7 positive group （t=3.23, P〈0.01; t=3.89, P〈 0.01）. Conclusion VEGF and MMP-7 expression are associated with high angiogenesis in colorectal cancer, accordingly VEGF and MMP-7 may serve as new target proteins to prevent the invasion and metastasis of colorectal cancer.
出处
《中国医药》
2009年第4期289-291,共3页
China Medicine
关键词
大肠癌
血管内皮生长因子
基质金属蛋白酶7
微血管密度
Colorectal cancer
Vascular endothelial growth factor
Matrix metalloproteinases-7
Microvessel density
