摘要
目的:利用基因芯片技术研究运动对雌性生长期大鼠长骨骨代谢相关基因表达的影响,以揭示运动对长骨生长影响的分子生物学机制;研究方法:雌性5周龄大鼠16只,随机分为对照组和跑台运动组,跑台运动组进行每周5天,每天2次的9周跑台运动,运动负荷递增,于第4周达到并维持每次15min,中间间隔30min的40m/min跑台运动。随机选取3只运动组大鼠股骨,以对照组大鼠股骨为参照进行3张基因芯片的测定;结果:运动组大鼠股骨干骺端Alp1、Thbs4、Col5a2、Spp1、Sparc、Fn1等mRNA表达上调,Timp1和Atp6v1g1等mRNA表达下调;结论:9周大强度间歇跑台运动对成骨细胞的分化和增殖具有一定的促进作用,对骨形成的骨基质形成阶段和基质钙化阶段均有促进作用,抑制骨基质分解和破骨细胞的骨吸收作用,因此,大强度间歇运动可以通过促进骨形成、抑制骨吸收而提高骨量的累积。
The purpose of this study is to examine the effects of exercise on the alterations in gene expression profile associated with bone metabolism and investigate the molecular mechanisms of exercise in pubertal bones. Method: 16 female SD rats (5-week-old) were randomly assigned to control group and exercise group. The exercise group underwent 9 weeks treadmill running exercise in 2 bouts separated by a 30-minute interval each day, 5 times a week. The alterations in gene expression profile of the femora were examined by gene chips. The data suggests that exercise can up-regulate mRNA expression of proteins generated by osteoblasts in its different development stages and stimulate the generation and mineralization of bone matrix. At the same time, exercise inhabits bone absorption by reducing the generation of V-ATPase.
出处
《体育科学》
CSSCI
北大核心
2009年第4期51-54,共4页
China Sport Science
基金
江苏省教育厅自然科学基金项目(06KJD330183)
关键词
生长期
跑台运动
骨代谢
基因表达
鼠
动物实验
treadmill running
growing rats
bone metabolism
gene expression
rat
animal experiment