摘要
本研究去除了猪繁殖与呼吸综合征病毒(PRRSV)CH-1a株M蛋白基因部分疏水性区域后,克隆于原核表达载体pGEX-6P-1中进行表达,并利用表达的M蛋白(rtM)为抗原,免疫BALB/c小鼠制备了1株抗M蛋白的单克隆抗体(McAb)M2B3,IFA试验表明该McAb能够识别PRRSVCH-1a株。同时将M蛋白基因进行一系列的氨基酸截短表达,证实M蛋白的117aa～129aa是McAbM2B3的抗原表位;对该抗原表位进行western blot分析,结果显示该表位能被PRRSV感染猪的血清特异性识别。本研究结果为进一步深入了解M蛋白的抗原特性奠定了基础。
A B-cell antigenic epitope was identified on the M protein of porcine, reproductive and respiratory syndrome virus （PRRSV） using a McAb M2B3. The M2B3 antibody was generated from the M protein with hydrophobic region deleted, which recognises both classical PRRSV CH-1a strain and highly pathogenic PRRSV HuN4 strain. To map the antigenic epitope, the M protein was truncated into two overlapping fragments （M1 and M2） and expressed. Both the truncated proteins could be recognised by M2B3. The overlapping region of M1 and M2 was then further truncated into a series of smaller fragments from amino terminal or carboxylic terminal, and a peptide 117 aa-129 aa was identified as the antigenic epitope for M2B3. This epitope is relatively conserved in north American isolates and could be recognized by serum from pigs infected by PRRSV.
出处
《中国预防兽医学报》
CAS
CSCD
北大核心
2009年第4期251-255,共5页
Chinese Journal of Preventive Veterinary Medicine
基金
黑龙江省政府博士后基金
国家“973”计划(2005CB523200)
十一五国家科技支撑计划(2006BAD06A04)
国家自然科学基金(30600443)
关键词
猪繁殖与呼吸综合征病毒
M蛋白
单克隆抗体
抗原表位
porcine reproductive and respiratory syndrome virus
M protein
McAb
antigenic epitope
