摘要
正常体外培养的细胞在体外有一定的寿命,一般传代不超过50代,被称为有限细胞系,但有些体外培养的细胞可经过自发或外界因素的影响从凋亡危机中逃离出来,从而形成具有无限增殖能力的细胞系,成为永生化细胞系。由于肝细胞在体外增殖能力极弱,培养困难,其应用受到较大限制,而肝细胞经永生化后具有了无限增殖优势,使其在肝细胞移植,生物人工肝以及药理、毒理学等研究方面有广阔的应用前景。就近年来肝细胞永生化方法及其应用作简要的综述。
Normal cultured cells in vitro have limited life span in which its general passage is no more than 50 generations that has been called as finite cell lines. However, some cultured cells can escape from the proliferation crisis spontaneously effected by several external factors to form a limitless proliferation cell lines, which is called as immortalized cell lines. Because the proliferation of hepatocytes in vitro is extremely weak and difficult to be cultivated,thus applications have to be limited. With the advantage of unlimit- ed proliferation, immortalized hepatocytes have been broadly used in the hepatocytes transplantation, bio-artificial liver and pharmacology as well as toxicology studies. In this paper, a brief overview on immortalized hepatocytes and its applications was given.
出处
《生物技术通报》
CAS
CSCD
北大核心
2009年第4期32-35,共4页
Biotechnology Bulletin
基金
国家重大基础研究“973”课题(2009CB522502)
云南省科技平台项目(2006PT04,2006PT07-2)
关键词
永生化
肝细胞
SV40
端粒酶
可恢复性永生化
肝细胞移植
生物人工肝
Immortalization Hepatocytes Simian virus 40 Telomerase Reversible immortalization Hepatocytes transplantation Bio-artificial liver
