摘要
目的:探讨Raf-1在肝细胞肝癌组织中表达的临床意义、相关性及对预后的影响.方法:应用免疫组织化学方法检测50例肝细胞肝癌组织、17例癌旁硬化肝组织及14例正常肝组织Raf-1的表达.分析其与临床特征的关系.结果:Raf-1在肝细胞肝癌组织中的表达显著高于癌旁硬化肝组织和正常肝组织(Z=-5.079,Z=-5.082,均P=0.000).Raf-1表达与临床分期、病理分级、年龄、肿瘤大小、癌栓明显相关(r=-0.452,-0.547,0.301,-0.357,-0.464,均P<0.05).Raf-1表达(-)-(+)者平均生存时间较短,生存率较低,预后较差;Raf-1表达(++)-(+++)者平均生存时间较长,生存率较高,预后较好.结论:Raf-1在肝细胞肝癌组织中的表达显著升高.对伴有癌栓、肿瘤较大且分化较差、属临床分期较晚的青壮年肝癌患者,Raf-1阳性表达较弱,生存期较短,预后较差.
AIM： To study the relationship between Raf-1 levels predicts and clinical outcome of hepatocellular carcinoma （HCC）.METHODS： Immunohistochemistry method was used to stain slide of 50 HCC tissue, 17 corresponding non-cancerous tissue and 14 normal hepatic tissue for Raf-1 protein, and analysis the relationship between expression of Raf-1 and clinical outcome.RESULTS： Raf-1 expressional frequency in HCC was significantly higher than that in adjacent non-cancerous hepatic tissue and in normal hepatic tissue （Z = -5.079, Z = -5.082, both P = 0.000）. The expression of Raf-1 was relation with clinical stage, pathology classification, age, tumor size and cancer bolt （r = -0.452, -0.547, 0.301,-0.357, -0.464, all P 〈 0.05）. Raf-1 was less stained in As for tumor tissues, the tissue with later clinical stage and shorter survival, moderately and strongly stained in the tissue with earlier clinical stage and longer survival.CONCLUSION： Raf-1 expressional levels were higher in tumor tissues, while positively corre- lated with survival rate and clinical stage.
出处
《世界华人消化杂志》
CAS
北大核心
2009年第6期618-622,共5页
World Chinese Journal of Digestology
基金
河北省科技厅科技攻关计划基金资助项目
No.052761260~~
