摘要
目的探讨双歧杆菌表面分子完整肽聚糖(whole peptidoglycan,WPG)的抗肿瘤作用及其机制。方法体外培养大肠癌Lovo细胞,应用MTT法、流式细胞术观察WPG对大肠癌Lovo细胞的增生抑制作用及对细胞的凋亡诱导作用。结果经WPG处理过的大肠癌Lovo细胞生长被抑制,且具有剂量和时间依赖性;早期凋亡细胞明显增加,与空白组比较有显著性差异(P<0.01)。结论WPG能够抑制大肠癌Lovo细胞的增生,其机制与诱导癌细胞早期凋亡有关。
Objective To explore the antitumor activities and mechanisms of bifid bacterial surface molecules, whole peptidoglycan （WPG）. Methods First, large intestine cancer Lovo cell was cultured in vitro. Then, anti -proliferative effect and apoptosis inducing effect of WPG to LoVo cell were observed respectively by MTT assay and flow cytometry. Results In the group treated with WPG, the growth of LoVo cell was inhibited significantly in a dose - and time - dependent manner ; early apoptotic cells were greatly increased, and compared with the blank control group, a big difference existed（P 〈 0.01 ）. Conclusion WPG has the growth inhibition to LoVo cell and its mechanism is interrelated to the early apoptosis of inducing cuneer cell.
出处
《医学研究杂志》
2009年第4期81-82,F0003,共3页
Journal of Medical Research
基金
黑龙江省教育厅科学技术研究计划(11531429)
关键词
双歧杆菌
完整肽聚糖抗肿瘤
诱导作用
早期凋亡
Bifid bacterium
Whole peptidoglycan
Anti -tumor
Inducing effect
Early apoptosis
