摘要
目的探讨全反式维甲酸(ATRA)对结肠癌Lovo细胞血管内皮细胞生长因子(VEGF)及其受体的影响,为其临床应用提供理论依据。方法 MTT法观察不同浓度ATRA对结肠癌Lovo细胞株生长的抑制作用以及对外源性VEGF刺激Lovo细胞生长的抑制作用。流式细胞仪分析ATRA作用后Lovo细胞周期变化以及细胞凋亡情况。ELISA法检测ATRA作用前后细胞培养上清液中VEGF含量变化。流式细胞仪测定Lovo细胞VEGF受体表达。结果 ATRA对Lovo细胞的生长具有抑制作用,呈时间与剂量依赖性。外源性VEGF165能刺激Lovo细胞生长,ATRA能够抑制VEGF165对细胞生长的刺激作用。随ATRA作用浓度增加,细胞周期G0/G1期细胞比例从(60.10±1.27)%增加至(84.80±1.40)%;细胞凋亡率增加至(39.79±3.96)%。ATRA能抑制Lovo细胞表达VEGF及VEGF受体,呈时间与剂量依赖性。结论 ATRA具有抑制结肠癌Lovo细胞株VEGF及其受体表达的作用,抑制肿瘤细胞的生长,可能机制为阻断VEGF自分泌及旁分泌,与促进肿瘤细胞凋亡及阻滞细胞周期有关。
Objective To investigate the effect of all-trans retinoic acid(ATRA) on the expressions of VEGF and its receptors,and growth inhibition of Lovo cells,and analyze its possible mechanisms.Methods Lovo cells were treated with ATRA at different concentrations for different times,and MTT assay was used to measure cell growth inhibition.The growth stimulation of exogenous recombinant human VEGF165 to Lovo cells and its inhibitory effect of ATRA were also examined.Cell cycle and apoptosis were evaluated by flow cytometry(FCM).The level of VEGF secreted by Lovo cells and expressions of VEGF receptors were measured using ELISA technique and flow cytometry respectively..Results ATRA greatly inhibited the proliferation of Lovo cells in dose-and time-dependent manners.Exogenous recombinant human VEGF165 stimulated Lovo cell growth and such growth stimulation was inhibited dose-dependently by ATRA.With the increasement of ATRA concentrtation,the proportion of cell cycle G0/G1 cells increased from(60.10±1.27)% to(84.80±1.40)%,and the apoptosis rate increased to(39.79±3.96)%.The level of VEGF secreted by Lovo cells and expressions of its receptors were decreased with ATRA treatment..Conclusions ATRA decreases the expressions of VEGF and its receptors,and inhibits tumor cell growth.The possible mechanisms may be involved in blockade of the paracrine and autocrine pathways of VEGF,acceleration of tumor cell apoptosis and blockade of cell cycle.
出处
《消化肿瘤杂志（电子版）》
2010年第3期145-148,共4页
Journal of Digestive Oncology(Electronic Version)
基金
国家自然科学基金资助项目(编号30872462)
广东省科技计划项目(编号2008B030301130)
关键词
全反式维甲酸
LOVO细胞株
血管内皮生长因子
细胞周期
细胞凋亡
All-trans retinoic acid
Lovo cells
Vascular endothelial growth factor
Vascular endothelial growth factor receptor
