摘要
目的:探讨小剂量全反式维甲酸(ATRA)对人结肠癌LoVo细胞短期作用的效果和意义。方法:MTT法筛选ATRA实验浓度,用筛选浓度ATRA作用LoVo细胞,MTT法检测ATRA对肿瘤细胞的抑制率,流式细胞仪检测ATRA对肿瘤细胞的细胞周期和凋亡率改变。结果:ATRA抑制LoVo细胞的作用呈时效和量效依赖性,1μmol·L^(-1)ATRA作用48 h或72 h可明显抑制肿瘤细胞增殖,与作用12 h或24 h对肿瘤细胞的抑制率有显著差异(P<0.01);1.0μmol·L^(-1)ATRA作用12 h,肿瘤细胞G_1期比例升高,其作用24 h伴细胞S期比例降低,其作用48 h,伴细胞G_2/M期比例降低(P<0.01);1μmol·L^(-1)ATRA作用48 h或72 h均可明显诱导肿瘤细胞凋亡,但对肿瘤细胞的抑制率、细胞周期和凋亡率影响无差异(P>0.01)。结论:小剂量/短程ATRA通过改变LoVo细胞周期和诱导细胞凋亡,可明显抑制肿瘤细胞增殖。
Objective:To investigate roles of a low-dose and short-term ATRA(All-trans retinoic acid) in human colorectal cancer cell line LoVo.Methods:The drug concentration of ATRA in LoVo cells were determined by MTT screen assay.LoVo cells were cultured at the screened concentration.Growth inhibitory activity against LoVo cells was determined by MTT assay.Cell cycle and apoptosis induced by ATRA was assessed by flow cytometry. Results:In LoVo cells,ATRA was a dose- and time-dependent drug,which had significant growth inhibitory activity against LoVo cells after 48 h or 72 h continuous treatment at 1μmol·L^(-1).The cells in G_1-phase began to increase after 12 h ATRA treatment at 1 μmol·L^(-1) and the cells in S-phase decreased significantly after 24 h and done with the cells in G_2/M phase after 48(?) h treatment.ATRA can significantly induce LoVo cells early apoptosis after 48 h or 72 h continuous treatment at 1 μmol·L^(-1).The inhibited growth,induced early apoptosis rate and changed cell cycle of LoVo cells after 48 h treatment were the same as that after 72 h treatment.Conclusion:A low-dose and short-term ATRA can significantly inhibit growth of LoVo cells by changing cell cycle and inducing apoptosis.
出处
《岭南急诊医学杂志》
2009年第3期180-181,173,共3页
Lingnan Journal of Emergency Medicine
关键词
全反式维甲酸
人结肠癌细胞
药物剂量
all-trans retinoic acid
human colon cancer cell line
drug dose
