摘要
目的:探讨冰冻切片制作方法,以提高冰冻切片的质量,确保实验结果科学性。方法:首先详细了解患者临床情况及既往有关的病理学检查情况,选择新鲜活体组织,取材后直接冷冻、切片,切片制成后,立即放入甲醇中固定10 s^20 s。染色1~2m in,水洗,逐级梯度乙醇脱水,二甲苯透明,中性树胶封固。结果:此方法简单,制片时间短,切片质量较好,染色鲜艳,镜下组织结构、切片组织切面完整、无裂纹;细胞形态清晰,细胞核、细胞浆染色佳,术后诊断符合率达96%以上。结论:冰冻切片是较难掌握的病理实验技术之一,具有简便快速,制片质量高等特点,可提高病理诊断的准确性。
Objective To evaluate the clinical effect and mechanism of arsenic trioxide（As2O3）in the treatment of primary hepatocellular carcinoma.MethodFifty-three patients with primary hepatocellular carcinoma hospitalized from January 2002 to January 2004 were studied（male 37,female 16,age 35～72 years old,average 55.7 years old）.In the situation of agreement,20mg As2O3 was given by artery perfusion qd for 5 days each treatment course and was repeated for 6 times.Every month they were examined by B ultrasound or CT.ResultsAfter 6 treatment courses of As2O3,the tumor volume of 38 patients reduced or invariable,the efficiency was 71.6%.Forty-eight patients were made a follow-up visit,the longest survival time was 23 months,a year survival rate was 60.2%.ConclusionAs2O3 artery perfusion therapy has a good clinical effect in the treatment of primary hepatocellular carcinoma which is unfit for operation.
出处
《吉林医学》
CAS
2009年第6期493-494,共2页
Jilin Medical Journal
关键词
病理
冰冻切片
制作方法
Pathology
Frozen section
Production method
