摘要
微芯片技术是基于微机电加工技术(MEMS)工艺,在芯片上完成电泳检测过程的新型技术.利用自制的微流控芯片及激光诱导荧光系统建立了痕量乳酸脱氢酶(LDH)活性的检测方法.以pH9.4,75mmol/L硼酸为芯片电泳缓冲液,9.73μmol/L乳酸钙为添加剂,整个电泳过程4min结束,利用该法测得LDH检测限(S/N=3)为6×10-3U/L,出峰时间和峰面积的相对标准偏差分别为5.32%和3.17%,该法操作过程简单,检测灵敏度高,在临床痕量酶检测中具有较好的应用前景.
The microchip fabricated by MEMS technology is successfully used to finish the electrophoresis process. A novel electrophoresis analysis protocol of the trace enzyme of lactate dehydrogenase （LDH） on microfluidic chip platform is developed with a Xe lamp-induced fluorescence detection system. Satisfactory separation of LDH was achieved in 75 mmol/L borate buffer containing 9.73 ixmol/L calcium lactate as running buffer （pH 9.4） within 4 rain. The detection of LDH limits （S/N=3） is 6×10^-3 U/L. The coefficients of variation of peak time and areas were 5.32% and 3.17%. The method is easily operated. To application microfluidic chips with the method, the sensitive of detectable enzyme will be much more improved. The method for trace enzymes has potential for clinical application.
出处
《生物化学与生物物理进展》
SCIE
CAS
CSCD
北大核心
2009年第4期512-516,共5页
Progress In Biochemistry and Biophysics
基金
国家高技术研究发展计划(863)资助项目(2003AA404200)~~
关键词
芯片毛细管电泳
氧化型辅酶Ⅰ
还原型辅酶Ⅰ
乳酸脱氢酶
microchip capillary electrophoresis, nicotinamide adenine dinucleotide（NAD＋）, reduced nicotinamide adenine dinucleotide（NADH）, lactate dehydrogenase
