摘要
目的观察槲皮苷防治溃疡性结肠炎及抗炎、镇痛、止泻等药效。方法以醋酸灌肠复制溃疡性结肠炎模型,大鼠从造模前2d至造模后6dig给予槲皮苷(50,200mg·kg-1·d-1),取结肠粘膜进行组织病理学检查以及丙二醛(MDA)、髓过氧化物酶(MPO)测定。小鼠ig给予槲皮苷(75,300mg·kg-1·d-1)4d后测定腹腔毛细血管通透性、醋酸ip引起扭体反应以及番泻叶煎剂ig引起腹泻潜伏期及次数变化。结果槲皮苷减轻溃疡性结肠炎大鼠结肠病变程度,降低结肠粘膜组织MDA及MPO水平(P<0.05);抑制醋酸引起的小鼠腹腔毛细血管通透性增加,减少扭体次数(P<0.05);对腹泻潜伏期及腹泻次数无显著影响(P>0.05)。结论槲皮苷对大鼠溃疡性结肠炎具有防治作用,并有抗炎、镇痛作用。
OBJECTIVE To investigate the protective effect of Quercitrin against ulcerative colitis in rats and anti-inflammatory, analgesic and anti-diarrhea effects of Quercitrin in mice. METHODS Rats treated with oral Quercitrin （50,200mg·kg^-1·d^-1） for 2 days before and 6 days after ulcerative colitis induced by acetic acid. Colonic pathological changes, tissue malondialdehyde （MDA） and myeloperoxidase （MPO） levels were determined. Anti-inflammatory, analgesic and anti-diarrhea effects of Quercitfin （75,300 mg·kg^-1·d^-1） treated with oral for 4 days in mice were determined by penetrability of abdominal capillary vessel assay, acetic acid-induced writhing assay and senna-induced diarrhea assay respectively. RESULTS Quercitfin improved colonic pathological changes and reduced MDA and MPO levels in ulcerative colitis rats （ P 〈 0.05 ）. Quercitrin suppressed the increased penetrability of abdominal capillary vessel induced by acetic acid （ P 〈 0.05 ） ; inhibited the writhing response induced by acetic acid （P 〈 0.05 ） ; but had no significant effect on diarrhea induced by senna in mice （ P 〉 0.05 ）. CONCLUSION Quercitrin has the protective effect against ulcerative colitis in rats and has anti-inflammatory and analgesic effects in mice.
出处
《中国现代应用药学》
CAS
CSCD
北大核心
2009年第3期180-184,共5页
Chinese Journal of Modern Applied Pharmacy
基金
浙江省医学科学院青年科研启动经费A10505Q
关键词
槲皮苷
溃疡性结肠炎
抗炎
镇痛
止泻
Quercitrin
ulcerative colitis
anti-inflammatory
analgesic
anti-diarrhea
