摘要
目的通过对肿瘤患者尿液标本中氨基酸代谢衍生物进行检测，探讨其在肿瘤筛查及判断手术疗效和预后中的可行性.方法采用癌症尿液筛查监测试剂（URC）分别对300例癌症患者、350例正常对照尿液标本进行检测，通过观察其反应沉淀物的颜色并与标准相对照来定性判断标本中氨基酸代谢物的含量;并对其中肝癌患者血清进行甲胎蛋白（AFP）含量检测以判断URC与AFP在诊断肝癌中的一致性.结果正常对照组中，URC的阳性检出率为2．86%，癌症组中阳性检出率为98．67%，方法特异性为97．14%;肝癌患者手术前后的阳性率分别为100%和23．71%，手术前阳性率显著高于手术后（P〈0．01）;消化道恶性肿瘤患者URC检测阳性率较非消化道肿瘤高（P〈0．01）.血清AFP与尿URC检测的结果具有高度一致性（Kappa=0．789）.结论利用URC来时尿液中肿瘤代谢物进行检测是一种可行的肿瘤初步诊断方法，可用于临床大规模的癌症患者筛查或集体查体，也适用于基层医院.
Objective To validate the feasibility of diagnosing and screening for cancer by detecting urinary amino acid derivatives. Methods Urine test reagents for cancer screen and monitoring （URC） were applied to detect samples from 300 carcinoma patients and 350 healthy individuals respectively. Then the change of the urinary color was observed for qualitative analysis of certain amino acid and their derivates. Also the serum AFP concentration was evaluated to discuss the correlation between AFP and URC method in diagnosing hepatocellular carcinoma. Results The positive rate was 2. 86% in control group,and 98.67% in cancer group. The sensitivity and specificity of the method were 98. 67% and 97. 14% respectively. The positive rates of hepatocellular carcinoma patients of before and after operation were 100% and 23. 71% respectively,which had significantly decreased with the operation procedure （P〈0.01）. The positive rate in digestive system was much higher than in others tissues （P〈0.01）. The results from serum AFP and urine URC were highly related（Kappa=0. 789）. Conclusion Using URC to detect the materials derivated from amino acid in urine samples is a feasible method for cancer screening,and it can be applied in large scale screening and periodic physical examination, as well as in small scale hospitals.
出处
《现代检验医学杂志》
CAS
2009年第2期66-69,共4页
Journal of Modern Laboratory Medicine
关键词
氨基酸
癌症
诊断
代谢组学
amino acid
carcinoma
diagnosis
metabolomics
