摘要
为筛选六月雪中齐墩果酸的最佳提取工艺,采用L9(34)正交设计考察药材粒度、乙醇用量、乙醇体积分数和提取次数4个因素,用高效液相色谱法测定六月雪中齐墩果酸的含量。结果表明,六月雪中齐墩果酸的最佳提取工艺为A3B2C2D1,即90目的药材,加入12倍的70%的乙醇水浴回流1次(2h),齐墩果酸的提取率为0.9980‰。经检验,本试验所优选的工艺合理,齐墩果酸质量稳定,适宜于工业化生产。
To optimize the extraction conditions of oleanolic acid from Serissa Serissoides,herbal granularity,ethanol usage, ethanol concentration and extraction times were take as evaluation factors by orthogonal test L9 （3^4）. The content of oleanolic acid was determined by HPLC. The results showed that optimum conditions of extraction were A3B2C2D1 as follows： 90 mesh herbal powder, 12-fold 70% ethanol and one time reflux for 2 hours.
出处
《湖北农业科学》
北大核心
2009年第2期425-426,共2页
Hubei Agricultural Sciences
基金
赣南医学院院级科研课题(2007124)
关键词
六月雪
齐墩果酸
提取工艺
正交设计
Serissa serissoides （DC.） Druce
oleanlic acid
extraction technique
orthogonal test
